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Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain

Mouille, G., Ralet, M.C., Cavelier, C., Eland, C., Effroy, D., Hematy, K., McCartney, L., Truong, H.N., Gaudon, V., Thibault, J.F., Marchant, A. and Hofte, H. (2007) Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain Plant Journal, 50, (4), pp. 605-614. (doi:10.1111/j.1365-313X.2007.03086.x).

Record type: Article

Abstract

Pectins are a family of complex cell-wall polysaccharides, the biosynthesis of which remains poorly understood. We identified dwarf mutants with reduced cell adhesion at a novel locus, QUASIMODO2 (QUA2). qua2-1 showed a 50% reduction in homogalacturonan (HG) content compared with the wild type, without affecting other cell-wall polysaccharides. The remaining HG in qua2-1 showed an unaltered degree of methylesterification. Positional cloning and GFP fusions showed that QUA2, consistent with a role in HG synthesis, encodes a Golgi-localized protein. In contrast to QUA1, another Golgi-localized protein required for HG-synthesis, QUA2 does not show sequence similarity to glycosyltransferases, but instead contains a putative methyltransferase (MT) domain. The Arabidopsis genome encodes 29 QUA2-related proteins. Interestingly, the transcript profiles of QUA1 and QUA2 are correlated and other pairs of QUA1 and QUA2 homologues with correlated transcript profiles can be identified. Together, the results lead to the hypothesis that QUA2 is a pectin MT, and that polymerization and methylation of homogalacturonan are interdependent reactions.

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Submitted date: 2 November 2006
Published date: 1 May 2007

Identifiers

Local EPrints ID: 56484
URI: http://eprints.soton.ac.uk/id/eprint/56484
ISSN: 0960-7412
PURE UUID: ff5dabf9-0ffe-4f66-8cfb-4f584333efc9

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Date deposited: 08 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: G. Mouille
Author: M.C. Ralet
Author: C. Cavelier
Author: C. Eland
Author: D. Effroy
Author: K. Hematy
Author: L. McCartney
Author: H.N. Truong
Author: V. Gaudon
Author: J.F. Thibault
Author: A. Marchant
Author: H. Hofte

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