The University of Southampton
University of Southampton Institutional Repository

The Ca(v)2.3 Ca2+ channel subunit contributes to R-type Ca2+ currents in murine hippocampal and neocortical neurones

Sochivko, D., Pereverzev, A., Smyth, N., Gissel, C., Schneider, T. and Beck, H. (2002) The Ca(v)2.3 Ca2+ channel subunit contributes to R-type Ca2+ currents in murine hippocampal and neocortical neurones The Journal of Physiology, 542, (3), pp. 699-710. (doi:10.1113/jphysiol.2002.020677).

Record type: Article


Different subtypes of voltage-dependent Ca2+ currents in native neurones are essential in coupling action potential firing to Ca2+ influx. For most of these currents, the underlying Ca2+ channel subunits have been identified on the basis of pharmacological and biophysical similarities. In contrast, the molecular basis of R-type Ca2+ currents remains controversial. We have therefore examined the contribution of the CaV2.3 (alpha1E) subunits to R-type currents in different types of central neurones using wild-type mice and mice in which the CaV2.3 subunit gene was deleted. In hippocampal CA1 pyramidal cells and dentate granule neurones, as well as neocortical neurones of wild-type mice, Ca2+ current components resistant to the combined application of omega-conotoxin GVIA and MVIIC, omega-agatoxin IVa and nifedipine (ICa,R) were detected that were composed of a large R-type and a smaller T-type component. In CaV2.3-deficient mice, ICa,R was considerably reduced in CA1 neurones (79 %) and cortical neurones (87 %), with less reduction occurring in dentate granule neurones (47 %). Analysis of tail currents revealed that the reduction of ICa,R is due to a selective reduction of the rapidly deactivating R-type current component in CA1 and cortical neurones. In all cell types, ICa,R was highly sensitive to Ni2+ (100 µM: 71-86 % block). A selective antagonist of cloned CaV2.3 channels, the spider toxin SNX-482, partially inhibited ICa,R at concentrations up to 300 nM in dentate granule cells and cortical neurones (50 and 57 % block, EC50 30 and 47 nM, respectively). ICa,R in CA1 neurones was significantly less sensitive to SNX-482 (27 % block, 300 nM SNX-482). Taken together, our results show clearly that CaV2.3 subunits underlie a significant fraction of ICa,R in different types of central neurones. They also indicate that CaV2.3 subunits may give rise to Ca2+ currents with differing pharmacological properties in native neurones.

Full text not available from this repository.

More information

Published date: 1 August 2002
Organisations: Biological Sciences


Local EPrints ID: 56486
ISSN: 0022-3751
PURE UUID: 066725be-ab81-4ac7-ab58-a8f32cfdb9f7

Catalogue record

Date deposited: 08 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record



Author: D. Sochivko
Author: A. Pereverzev
Author: N. Smyth
Author: C. Gissel
Author: T. Schneider
Author: H. Beck

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.