The University of Southampton
University of Southampton Institutional Repository

Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor

Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor
Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor
Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 {(2R,3S)-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate} for X-ray crystallography. Tetragonal complex crystals with space group P43212 were produced by the hanging-drop vapour-diffusion method and diffracted to 3.0 Å. The crystals exhibited unit-cell parameters a = b = 182.5, c = 99.1 Å and contained four molecules in the asymmetric unit. A 96% complete data set was collected at 298 K using Cu K X-rays from a rotating-anode generator. Solution of the crystal structure of Y75N mutant M. pusillus pepsin is under way by molecular replacement using the molecular coordinates of wild-type M. pusillus pepsin as a model.
pepsins, aspartic proteinases, inhibitors
0907-4449
770-772
Badasso, M.O.
59a5d949-d29d-4be7-ab09-930dcf76d889
Dhanaraj, V.
60666c14-e81e-47cf-ba59-a18d5d98ccd8
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0
Blundell, T.L.
7311fa34-6cbd-45da-ba4e-cfdafe447180
Badasso, M.O.
59a5d949-d29d-4be7-ab09-930dcf76d889
Dhanaraj, V.
60666c14-e81e-47cf-ba59-a18d5d98ccd8
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0
Blundell, T.L.
7311fa34-6cbd-45da-ba4e-cfdafe447180

Badasso, M.O., Dhanaraj, V., Wood, S.P., Cooper, J.B. and Blundell, T.L. (2004) Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor. Acta Crystallographica Section D: Biological Crystallography, 60, 770-772. (doi:10.1107/S0907444904003221).

Record type: Article

Abstract

Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 {(2R,3S)-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate} for X-ray crystallography. Tetragonal complex crystals with space group P43212 were produced by the hanging-drop vapour-diffusion method and diffracted to 3.0 Å. The crystals exhibited unit-cell parameters a = b = 182.5, c = 99.1 Å and contained four molecules in the asymmetric unit. A 96% complete data set was collected at 298 K using Cu K X-rays from a rotating-anode generator. Solution of the crystal structure of Y75N mutant M. pusillus pepsin is under way by molecular replacement using the molecular coordinates of wild-type M. pusillus pepsin as a model.

Full text not available from this repository.

More information

Published date: 1 April 2004
Keywords: pepsins, aspartic proteinases, inhibitors

Identifiers

Local EPrints ID: 56502
URI: https://eprints.soton.ac.uk/id/eprint/56502
ISSN: 0907-4449
PURE UUID: d5df9409-2aea-4143-80f0-2a8490767be9

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 13 Mar 2019 20:35

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×