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Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor

Record type: Article

Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 {(2R,3S)-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate} for X-ray crystallography. Tetragonal complex crystals with space group P43212 were produced by the hanging-drop vapour-diffusion method and diffracted to 3.0 Å. The crystals exhibited unit-cell parameters a = b = 182.5, c = 99.1 Å and contained four molecules in the asymmetric unit. A 96% complete data set was collected at 298 K using Cu K X-rays from a rotating-anode generator. Solution of the crystal structure of Y75N mutant M. pusillus pepsin is under way by molecular replacement using the molecular coordinates of wild-type M. pusillus pepsin as a model.

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Citation

Badasso, M.O., Dhanaraj, V., Wood, S.P., Cooper, J.B. and Blundell, T.L. (2004) Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor Acta Crystallographica Section D: Biological Crystallography, 60, pp. 770-772. (doi:10.1107/S0907444904003221).

More information

Published date: 1 April 2004
Keywords: pepsins, aspartic proteinases, inhibitors

Identifiers

Local EPrints ID: 56502
URI: http://eprints.soton.ac.uk/id/eprint/56502
ISSN: 0907-4449
PURE UUID: d5df9409-2aea-4143-80f0-2a8490767be9

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Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: M.O. Badasso
Author: V. Dhanaraj
Author: S.P. Wood
Author: J.B. Cooper
Author: T.L. Blundell

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