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Monocyte chemoattractant protein-1 deficiency is protective in a murine stroke model

Hughes, Paula M., Allegrini, Peter R., Rudin, Markus, Perry, V. Hugh, Mir, Anis K. and Wiessner, Christoph (2002) Monocyte chemoattractant protein-1 deficiency is protective in a murine stroke model Journal of Cerebral Blood Flow & Metabolism, 22, (3), pp. 308-317. (doi:10.1097/00004647-200203000-00008).

Record type: Article


Inflammatory processes have been implicated in the pathogenesis of brain damage after stroke. In rodent stroke models, focal ischemia induces several proinflammatory chemokines, including monocyte chemoattractant protein-1 (MCP-1). The individual contribution to ischemic tissue damage, however, is largely unknown. To address this question, the authors subjected MCP-1-deficient mice (MCP-1-/-) to permanent middle cerebral artery occlusion (MCAO). Measurement of basal blood pressure, cerebral blood flow, and blood volume revealed no differences between wild-type (wt) and MCP-1-/- mice. MCAO led to similar cerebral perfusion deficits in wt and MCP-1-/- mice, excluding differences in the MCA supply territory and collaterals. However, compared with wt mice, the mean infarct volume was 29% smaller in MCP-1-/- mice 24 hours after MCAO (P = 0.022). Immunostaining showed a reduction of phagocytic macrophage accumulation within infarcts and the infarct border in MCP-1-/- mice 2 weeks after MCAO. At the same time point, the authors found an attenuation of astrocytic hypertrophy in the infarct border and thalamus in MCP-1-/- mice. However, these effects on macrophages and astrocytes in MCP-1-/- mice occurred too late to suggest a protective role in acute infarct growth. Of note: at 6 hours after MCAO, MCP-1-/- mice produced significantly less interleukin-1beta in ischemic tissue; this might be related to tissue protection. The results of this study indicate that inhibition of MCP-1 signaling could be a new acute treatment approach to limit infarct size after stroke.

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Published date: March 2002
Keywords: MCP-1, chemokines, cytokines, neuroinflammation, focal cerebral ischemia, astrocytes, microglia


Local EPrints ID: 56503
ISSN: 0271-678X
PURE UUID: d112935e-517a-45aa-a403-6d63d6ab628a

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Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:30

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Author: Paula M. Hughes
Author: Peter R. Allegrini
Author: Markus Rudin
Author: V. Hugh Perry
Author: Anis K. Mir
Author: Christoph Wiessner

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