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Recruitment of neutrophils across the blood-brain barrier: the role of E- and P-selectins

Recruitment of neutrophils across the blood-brain barrier: the role of E- and P-selectins
Recruitment of neutrophils across the blood-brain barrier: the role of E- and P-selectins
The adult central nervous system parenchyma is resistant to inflammation, but in juvenile rats the injection of inflammatory mediators, interleukin-1beta for example, gives rise to extensive neutrophil recruitment and neutrophil-dependent blood–brain barrier breakdown. The factors that confer this resistant phenotype are unknown. In this study, the authors demonstrate that E- and P-selectin expression is increased to a similar extent in adult and juvenile brain after the intracerebral injection of IL-1beta. Thus, the refractory nature of the brain parenchyma cannot be attributed to an absence of selectin expression. However, in injuries where the resistant characteristic of the brain parenchyma is compromised, and neutrophil recruitment occurs, selectin blockade may be an advantage. The authors investigated the contribution that selectins make to neutrophil recruitment during acute inflammation in the brain. The authors examined neutrophil recruitment by immunohistochemistry on brain sections of juvenile rats killed four hours after the intracerebral injection of IL-1beta and the intravenous injection of neutralizing anti-selectin monoclonal antibodies (mAb). The administration of the P-selectin blocking mAb inhibited neutrophil recruitment by 85% compared with controls. Surprisingly, E-selectin blockade had no effect on neutrophil recruitment to the brain parenchyma. Thus, P-selectin appears to play a pivotal role in mediating neutrophil recruitment to the brain parenchyma during acute inflammation.
acute inflammation, blood-brain barrier, E-selectin, interleukin-1beta, neutrophils, P-selectin
0271-678X
1115-1124
Bernardes-Silva, Martine
513d0bcc-f15d-44d6-9373-a0d78b2b2e07
Anthony, Daniel C.
928249fa-dcf4-4088-b95b-ce14c719164d
Issekutz, Andrew C.
b40a2312-3464-47e8-ac3a-98d4e22dea9f
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Bernardes-Silva, Martine
513d0bcc-f15d-44d6-9373-a0d78b2b2e07
Anthony, Daniel C.
928249fa-dcf4-4088-b95b-ce14c719164d
Issekutz, Andrew C.
b40a2312-3464-47e8-ac3a-98d4e22dea9f
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4

Bernardes-Silva, Martine, Anthony, Daniel C., Issekutz, Andrew C. and Perry, V. Hugh (2001) Recruitment of neutrophils across the blood-brain barrier: the role of E- and P-selectins. Journal of Cerebral Blood Flow & Metabolism, 21 (9), 1115-1124. (doi:10.1097/00004647-200109000-00009).

Record type: Article

Abstract

The adult central nervous system parenchyma is resistant to inflammation, but in juvenile rats the injection of inflammatory mediators, interleukin-1beta for example, gives rise to extensive neutrophil recruitment and neutrophil-dependent blood–brain barrier breakdown. The factors that confer this resistant phenotype are unknown. In this study, the authors demonstrate that E- and P-selectin expression is increased to a similar extent in adult and juvenile brain after the intracerebral injection of IL-1beta. Thus, the refractory nature of the brain parenchyma cannot be attributed to an absence of selectin expression. However, in injuries where the resistant characteristic of the brain parenchyma is compromised, and neutrophil recruitment occurs, selectin blockade may be an advantage. The authors investigated the contribution that selectins make to neutrophil recruitment during acute inflammation in the brain. The authors examined neutrophil recruitment by immunohistochemistry on brain sections of juvenile rats killed four hours after the intracerebral injection of IL-1beta and the intravenous injection of neutralizing anti-selectin monoclonal antibodies (mAb). The administration of the P-selectin blocking mAb inhibited neutrophil recruitment by 85% compared with controls. Surprisingly, E-selectin blockade had no effect on neutrophil recruitment to the brain parenchyma. Thus, P-selectin appears to play a pivotal role in mediating neutrophil recruitment to the brain parenchyma during acute inflammation.

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More information

Published date: September 2001
Keywords: acute inflammation, blood-brain barrier, E-selectin, interleukin-1beta, neutrophils, P-selectin

Identifiers

Local EPrints ID: 56517
URI: http://eprints.soton.ac.uk/id/eprint/56517
ISSN: 0271-678X
PURE UUID: d1d5f50b-c162-48c1-9655-03e865199baf

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Date deposited: 07 Aug 2008
Last modified: 15 Mar 2024 11:02

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Contributors

Author: Martine Bernardes-Silva
Author: Daniel C. Anthony
Author: Andrew C. Issekutz
Author: V. Hugh Perry

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