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Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein

Record type: Article

Summary: Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked target sequences in the epidermis, in tongue, and thymic epithelium of the offspring where the Cre allele was inherited from the father. Where the mother carried the Cre allele, loxP flanked sequences were completely deleted in all tissues of the offspring, even in littermates that did not inherit the Cre allele. This maternally inherited phenotype indicates that the human K14 promoter is transcriptionally active in murine oocytes and that the enzyme remains active until after fertilization, even when the Cre allele becomes transmitted to the polar bodies during meiosis. Detection of K14 mRNA by RT-PCR in murine ovaries and immunohistochemical identification of the K14 protein in oocytes demonstrates that the human K14 promoter behaves like its murine homolog, thus identifying K14 as an authentic oocytic protein.

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Citation

Hafner, M., Wenk, J., Nenci, A., Pasparakis, M., Scharffetter-Kochanek, K., Smyth, N., Peters, T., Kess, D., Holtkotter, O., Shephard, P., Kudlow, J.E., Smola, H., Haase, I., Schippers, A., Krieg, T. and Muller, W. (2004) Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein Genesis, 38, (4), pp. 176-181. (doi:10.1002/gene.20016).

More information

Published date: 2 April 2004
Keywords: Keratin 14, intermediate filament, Cre recombinase, oocyte, maternal expression

Identifiers

Local EPrints ID: 56519
URI: http://eprints.soton.ac.uk/id/eprint/56519
ISSN: 1526-954X
PURE UUID: 5d747e95-1a84-4b21-813b-a55aecbab3a6

Catalogue record

Date deposited: 08 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: M. Hafner
Author: J. Wenk
Author: A. Nenci
Author: M. Pasparakis
Author: K. Scharffetter-Kochanek
Author: N. Smyth
Author: T. Peters
Author: D. Kess
Author: O. Holtkotter
Author: P. Shephard
Author: J.E. Kudlow
Author: H. Smola
Author: I. Haase
Author: A. Schippers
Author: T. Krieg
Author: W. Muller

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