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Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein

Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein
Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein
Summary: Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked target sequences in the epidermis, in tongue, and thymic epithelium of the offspring where the Cre allele was inherited from the father. Where the mother carried the Cre allele, loxP flanked sequences were completely deleted in all tissues of the offspring, even in littermates that did not inherit the Cre allele. This maternally inherited phenotype indicates that the human K14 promoter is transcriptionally active in murine oocytes and that the enzyme remains active until after fertilization, even when the Cre allele becomes transmitted to the polar bodies during meiosis. Detection of K14 mRNA by RT-PCR in murine ovaries and immunohistochemical identification of the K14 protein in oocytes demonstrates that the human K14 promoter behaves like its murine homolog, thus identifying K14 as an authentic oocytic protein.
Keratin 14, intermediate filament, Cre recombinase, oocyte, maternal expression
1526-954X
176-181
Hafner, M.
07c05f69-a9ef-4b5e-b771-2acecbb8a671
Wenk, J.
6430f41b-e02d-42e4-9983-7ce378b9d896
Nenci, A.
84a9508e-6fa7-40f0-901c-7bd69c618d9b
Pasparakis, M.
7444c519-cb49-4d05-8c19-8a5b5360182e
Scharffetter-Kochanek, K.
09df6148-f95c-4bfc-861c-7c3d12f362c4
Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Peters, T.
9aca9392-f159-4016-bf12-19a7b4b50c47
Kess, D.
ab4298a3-c3c7-445b-ad43-3e19a5e1ef15
Holtkotter, O.
3ad9f09d-cecb-418d-b812-c16ef2b5defd
Shephard, P.
c9e1a8be-3233-41ff-b239-9f3b3560ce8f
Kudlow, J.E.
95f32925-de92-4800-9bc0-f70072c54d54
Smola, H.
682c7e8d-5216-4efa-b965-03b654b3f304
Haase, I.
c21e0aea-e3a9-49cd-bdff-158b1909da7f
Schippers, A.
df6643ad-a9a0-4c4d-b298-b5aadde60407
Krieg, T.
7b2f2d7d-77df-4252-8629-53da67afe4cd
Muller, W.
51984881-dbe0-4c93-bf3d-bede3aff668f
Hafner, M.
07c05f69-a9ef-4b5e-b771-2acecbb8a671
Wenk, J.
6430f41b-e02d-42e4-9983-7ce378b9d896
Nenci, A.
84a9508e-6fa7-40f0-901c-7bd69c618d9b
Pasparakis, M.
7444c519-cb49-4d05-8c19-8a5b5360182e
Scharffetter-Kochanek, K.
09df6148-f95c-4bfc-861c-7c3d12f362c4
Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Peters, T.
9aca9392-f159-4016-bf12-19a7b4b50c47
Kess, D.
ab4298a3-c3c7-445b-ad43-3e19a5e1ef15
Holtkotter, O.
3ad9f09d-cecb-418d-b812-c16ef2b5defd
Shephard, P.
c9e1a8be-3233-41ff-b239-9f3b3560ce8f
Kudlow, J.E.
95f32925-de92-4800-9bc0-f70072c54d54
Smola, H.
682c7e8d-5216-4efa-b965-03b654b3f304
Haase, I.
c21e0aea-e3a9-49cd-bdff-158b1909da7f
Schippers, A.
df6643ad-a9a0-4c4d-b298-b5aadde60407
Krieg, T.
7b2f2d7d-77df-4252-8629-53da67afe4cd
Muller, W.
51984881-dbe0-4c93-bf3d-bede3aff668f

Hafner, M., Wenk, J., Nenci, A., Pasparakis, M., Scharffetter-Kochanek, K., Smyth, N., Peters, T., Kess, D., Holtkotter, O., Shephard, P., Kudlow, J.E., Smola, H., Haase, I., Schippers, A., Krieg, T. and Muller, W. (2004) Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein. Genesis, 38 (4), 176-181. (doi:10.1002/gene.20016).

Record type: Article

Abstract

Summary: Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked target sequences in the epidermis, in tongue, and thymic epithelium of the offspring where the Cre allele was inherited from the father. Where the mother carried the Cre allele, loxP flanked sequences were completely deleted in all tissues of the offspring, even in littermates that did not inherit the Cre allele. This maternally inherited phenotype indicates that the human K14 promoter is transcriptionally active in murine oocytes and that the enzyme remains active until after fertilization, even when the Cre allele becomes transmitted to the polar bodies during meiosis. Detection of K14 mRNA by RT-PCR in murine ovaries and immunohistochemical identification of the K14 protein in oocytes demonstrates that the human K14 promoter behaves like its murine homolog, thus identifying K14 as an authentic oocytic protein.

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More information

Published date: 2 April 2004
Keywords: Keratin 14, intermediate filament, Cre recombinase, oocyte, maternal expression

Identifiers

Local EPrints ID: 56519
URI: http://eprints.soton.ac.uk/id/eprint/56519
ISSN: 1526-954X
PURE UUID: 5d747e95-1a84-4b21-813b-a55aecbab3a6

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Date deposited: 08 Aug 2008
Last modified: 09 Jan 2022 07:24

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Contributors

Author: M. Hafner
Author: J. Wenk
Author: A. Nenci
Author: M. Pasparakis
Author: K. Scharffetter-Kochanek
Author: N. Smyth
Author: T. Peters
Author: D. Kess
Author: O. Holtkotter
Author: P. Shephard
Author: J.E. Kudlow
Author: H. Smola
Author: I. Haase
Author: A. Schippers
Author: T. Krieg
Author: W. Muller

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