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Effect of fatty acid supplementation on growth and differentiation of human IMR-32 neuroblastoma cells in vitro

Record type: Article

Polyunsaturated fatty acids play a critical role in the structure and function of the developing nervous system. It has been proposed that fatty acids may effect a variety of biologic processes through the activation of the peroxisome proliferator activated receptors (PPARs)-ligand activated transcription factors. In this report, we demonstrate that fatty acids can inhibit the proliferation of the human neuronal cell line IMR-32. The fatty acids linoleate, -linoleate, arachidonate, docosahexaenoate, and oleate all inhibited [3H]thymidine incorporation of IMR-32 cells after 72 h. Fatty acid supplementation also led to the morphologic differentiation of the IMR-32 cells. Linoleate and arachidonate, fatty acids of the n-6 series, induced the most extensive differentiation. Furthermore, the addition of fatty acids to IMR-32 cells led to PPAR activation, suggesting that PPAR activation may be an important event in fatty acid modulation of IMR-32 cell growth. In support of this hypothesis, clofibric acid, a specific ligand of PPAR, also inhibited IMR-32 cell proliferation and strongly induced PPAR activation. Together these results suggest that fatty acids may play an important role in the development of neuronal precursor cells and that activation of the PPARs may be one pathway by which fatty acids modulate the growth and differentiation of neuronal precursor cells. J. Cell. Biochem. 80:266-273, 2000.

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Citation

Burdge, G.C., Rodway, H., Kohler, J.A. and Lillycrop, K.A. (2000) Effect of fatty acid supplementation on growth and differentiation of human IMR-32 neuroblastoma cells in vitro Journal of Cellular Biochemistry, 80, (2), pp. 266-273. (doi:10.1002/1097-4644(20010201)80:2<266::AID-JCB160>3.0.CO;2-6).

More information

Published date: 2 November 2000
Keywords: neuroblastoma, fatty acid, differentiation, retinoic acid, peroxisomal proliferator activated receptor

Identifiers

Local EPrints ID: 56542
URI: http://eprints.soton.ac.uk/id/eprint/56542
ISSN: 0730-2312
PURE UUID: c2531183-a94e-4d01-bea1-409b4181c19b
ORCID for G.C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967
ORCID for K.A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489

Catalogue record

Date deposited: 21 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: G.C. Burdge ORCID iD
Author: H. Rodway
Author: J.A. Kohler
Author: K.A. Lillycrop ORCID iD

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