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Central nervous system injury triggers hepatic CC and CXC chemokine expression that is associated with leukocyte mobilization and recruitment to both the central nervous system and the liver

Central nervous system injury triggers hepatic CC and CXC chemokine expression that is associated with leukocyte mobilization and recruitment to both the central nervous system and the liver
Central nervous system injury triggers hepatic CC and CXC chemokine expression that is associated with leukocyte mobilization and recruitment to both the central nervous system and the liver
The administration of interleukin-1ß to the brain induces hepatic CXC chemokine synthesis, which increases neutrophil levels in the blood, liver, and brain. We now show that such hepatic response is not restricted to the CXC chemokines. CCL-2, a CC chemokine, was released by the liver in response to a tumor necrosis factor (TNF)- challenge to the brain and boosted monocyte levels. Furthermore, a clinically relevant compression injury to the spinal cord triggered hepatic chemokine expression of both types. After a spinal cord injury, elevated CCL-2 and CXCL-1 mRNA and protein were observed in the liver by TaqMan reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay as early as 2 to 4 hours. Simultaneously, we observed elevated levels of these chemokines and circulating leukocyte populations in the blood. Leukocytes were recruited to the liver at this early stage, whereas at the site of challenge in the central nervous system, few were observed until 24 hours. Artificial elevation of blood CCL-2 triggered dose-dependent monocyte mobilization in the blood and enhanced monocyte recruitment to the brain after TNF- challenge. Attenuation of hepatic CCL-2 production with corticosteroids resulted in reduced monocyte levels after the TNF- challenge. Thus, combined production of CC and CXC hepatic chemokines appears to amplify the central nervous system response to injury.
0002-9440
1487-1497
Campbell, S.J.
fe349c31-dac5-42fb-ae2c-0f841b145083
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Pitossi, F.J.
4bacb661-ef14-46c0-aba6-9f7fc3030904
Butchart, A.G.
ba2335d5-425a-4f69-ae0b-aa57118ac5d9
Chertoff, M.
41d84d18-bc31-478a-bcfb-817cfd9370bd
Waters, S.
7eefd97d-ab0d-437b-8935-deee6b3a85f8
Dempster, R.
5e22f8c5-f865-4422-98c8-cbf6a8c384df
Anthony, D.C.
70fb8e27-4e74-4c72-b1b5-3a4ca0d6b8cf
Campbell, S.J.
fe349c31-dac5-42fb-ae2c-0f841b145083
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Pitossi, F.J.
4bacb661-ef14-46c0-aba6-9f7fc3030904
Butchart, A.G.
ba2335d5-425a-4f69-ae0b-aa57118ac5d9
Chertoff, M.
41d84d18-bc31-478a-bcfb-817cfd9370bd
Waters, S.
7eefd97d-ab0d-437b-8935-deee6b3a85f8
Dempster, R.
5e22f8c5-f865-4422-98c8-cbf6a8c384df
Anthony, D.C.
70fb8e27-4e74-4c72-b1b5-3a4ca0d6b8cf

Campbell, S.J., Perry, V.H., Pitossi, F.J., Butchart, A.G., Chertoff, M., Waters, S., Dempster, R. and Anthony, D.C. (2005) Central nervous system injury triggers hepatic CC and CXC chemokine expression that is associated with leukocyte mobilization and recruitment to both the central nervous system and the liver. The American Journal of Pathology, 166, 1487-1497.

Record type: Article

Abstract

The administration of interleukin-1ß to the brain induces hepatic CXC chemokine synthesis, which increases neutrophil levels in the blood, liver, and brain. We now show that such hepatic response is not restricted to the CXC chemokines. CCL-2, a CC chemokine, was released by the liver in response to a tumor necrosis factor (TNF)- challenge to the brain and boosted monocyte levels. Furthermore, a clinically relevant compression injury to the spinal cord triggered hepatic chemokine expression of both types. After a spinal cord injury, elevated CCL-2 and CXCL-1 mRNA and protein were observed in the liver by TaqMan reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay as early as 2 to 4 hours. Simultaneously, we observed elevated levels of these chemokines and circulating leukocyte populations in the blood. Leukocytes were recruited to the liver at this early stage, whereas at the site of challenge in the central nervous system, few were observed until 24 hours. Artificial elevation of blood CCL-2 triggered dose-dependent monocyte mobilization in the blood and enhanced monocyte recruitment to the brain after TNF- challenge. Attenuation of hepatic CCL-2 production with corticosteroids resulted in reduced monocyte levels after the TNF- challenge. Thus, combined production of CC and CXC hepatic chemokines appears to amplify the central nervous system response to injury.

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Published date: 1 May 2005

Identifiers

Local EPrints ID: 56559
URI: https://eprints.soton.ac.uk/id/eprint/56559
ISSN: 0002-9440
PURE UUID: 68cc045d-90c1-40d6-a3cc-6fc6a711c801

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Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: S.J. Campbell
Author: V.H. Perry
Author: F.J. Pitossi
Author: A.G. Butchart
Author: M. Chertoff
Author: S. Waters
Author: R. Dempster
Author: D.C. Anthony

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