The University of Southampton
University of Southampton Institutional Repository

Overexpression of tau results in defective synaptic transmission in Drosophila neuromuscular junctions

Chee, F., Mudher, A., Newman, T.A., Cuttle, M., Lovestone, S. and Shepherd, D. (2006) Overexpression of tau results in defective synaptic transmission in Drosophila neuromuscular junctions Biochemical Society Transactions, 34, (1), pp. 88-90.

Record type: Article

Abstract

Synaptic dysfunction is believed to be an early pathological change in neurodegenerative diseases and may cause the earliest clinical symptoms. We have used Drosophila to model a tauopathy in order to analyse the earliest neuronal and synaptic dysfunction. Our work has shown that overexpression of human tau (0N3R) in larval motor neurons causes a disruption of axonal transport and a morphological and functional disruption of NMJs (neuromuscular junctions). Tau-expressing NMJs are smaller with an abnormal structure. Despite abnormal morphology, tau-expressing NMJs retain synaptotagmin expression and can form active zones. Tau-expressing NMJs are functionally abnormal and exhibit disrupted vesicle cycling and synaptic transmission. At low-frequency stimulation (1 Hz), ESPs (evoked synaptic potentials) produced by tau-expressing motor neurons were indistinguishable from wild-type; however, following high-frequency stimulation (50 Hz), ESPs from tau-expressing NMJs were significantly decreased in amplitude. To investigate the mechanism underlying the change in ESPs, we analysed the relative numbers and distribution of mitochondria. This revealed that motor neurons expressing tau had a significant reduction in the number of detectable mitochondria in the pre-synaptic terminal. Our results demonstrate that tau overexpression results in synaptic dysfunction, associated with a reduced complement of functional mitochondria. These findings suggest that disruption of axonal transport and synaptic transmission may be key components of the pathogenic mechanism that underlie neuronal dysfunction in the early stages of tauopathies.

Full text not available from this repository.

More information

Published date: 1 February 2006
Keywords: Alzheimer's disease, Drosophila, neurodegeneration, neuromuscular junction, synaptic transmission, tauopathy

Identifiers

Local EPrints ID: 56560
URI: http://eprints.soton.ac.uk/id/eprint/56560
ISSN: 0300-5127
PURE UUID: 770f7dd4-d320-428f-877e-167eae58f585

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record

Contributors

Author: F. Chee
Author: A. Mudher
Author: T.A. Newman
Author: M. Cuttle
Author: S. Lovestone
Author: D. Shepherd

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×