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Influence of the course of brain inflammation on the endogenous IL-1 beta/IL-1Ra balance in the model of brain delayed-type hypersensitivity response to bacillus Calmette-Guerin in Lewis rats

Influence of the course of brain inflammation on the endogenous IL-1 beta/IL-1Ra balance in the model of brain delayed-type hypersensitivity response to bacillus Calmette-Guerin in Lewis rats
Influence of the course of brain inflammation on the endogenous IL-1 beta/IL-1Ra balance in the model of brain delayed-type hypersensitivity response to bacillus Calmette-Guerin in Lewis rats
Interleukin-1? (IL-1?) is a key player in the pathogenesis of acute and chronic inflammatory diseases at the periphery and in the brain. Its action is regulated by interleukin-1 receptor antagonist (IL-1Ra), the specific endogenous antagonist of IL-1 receptors. The ratio between local concentrations of IL-1Ra and IL-1? is known to influence the initiation and progression of many inflammatory and autoimmune diseases at the periphery. In order to determine whether this is also the case in the brain, brain and plasma concentrations of IL-1? and IL-1Ra were measured by ELISA in a model of chronic brain inflammation in Lewis rats, the hippocampal delayed-type hypersensitivity (DTH) response to bacillus Calmette–Guérin (BCG). Brain IL-1? increased rapidly after intracerebral (i.c.) injection of BCG and came back to baseline concentrations 1 week later, whereas IL-1Ra increased gradually over time and remained elevated during the last 2 weeks post-BCG intracerebral injection. Following peripheral BCG challenge, brain IL-1? increased at the site of the brain BCG and peaked 12 days later before decreasing on day 16 post-challenge. Brain IL-1Ra remained elevated during the first days post-challenge and then decreased from the 12th day post-challenge. The same temporal variations were observed in the plasma concentrations of IL-1? and IL-1Ra. The increase in the IL-1?/IL-1Ra ratio that was apparent from day 3 to day 12 post-challenge might be correlated with the invasion of peripheral inflammatory cells at the site of intracerebral injection. Besides showing that the course of inflammation alters the brain IL-1?/IL-1Ra ratio, these findings point to the importance of monitoring plasma IL-1?/IL-1Ra ratio to predict the course of brain inflammation.
0165-5728
22-30
Palin, Karine
395fbcda-515f-4e3f-aac6-1c292d114a89
Verrier, Danièle
e1121680-b2e8-41e3-bc6e-eae7e82b2857
Tridon, Viviane
9ca6459b-2cf4-4ebd-b8a7-6414285d9405
Hurst, Jane
666ec21b-9ab9-4472-b55c-c64d9faa8695
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Dantzer, Robert
ef2dddbc-53b7-40f8-bdee-f28e8d8381b0
Lestage, Jacques
6f7d9c56-c14c-4318-9c03-70d2a249bd9e
Palin, Karine
395fbcda-515f-4e3f-aac6-1c292d114a89
Verrier, Danièle
e1121680-b2e8-41e3-bc6e-eae7e82b2857
Tridon, Viviane
9ca6459b-2cf4-4ebd-b8a7-6414285d9405
Hurst, Jane
666ec21b-9ab9-4472-b55c-c64d9faa8695
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Dantzer, Robert
ef2dddbc-53b7-40f8-bdee-f28e8d8381b0
Lestage, Jacques
6f7d9c56-c14c-4318-9c03-70d2a249bd9e

Palin, Karine, Verrier, Danièle, Tridon, Viviane, Hurst, Jane, Perry, V. Hugh, Dantzer, Robert and Lestage, Jacques (2004) Influence of the course of brain inflammation on the endogenous IL-1 beta/IL-1Ra balance in the model of brain delayed-type hypersensitivity response to bacillus Calmette-Guerin in Lewis rats. Journal of Neuroimmunology, 149 (1-2), 22-30. (doi:10.1016/j.jneuroim.2003.12.005).

Record type: Article

Abstract

Interleukin-1? (IL-1?) is a key player in the pathogenesis of acute and chronic inflammatory diseases at the periphery and in the brain. Its action is regulated by interleukin-1 receptor antagonist (IL-1Ra), the specific endogenous antagonist of IL-1 receptors. The ratio between local concentrations of IL-1Ra and IL-1? is known to influence the initiation and progression of many inflammatory and autoimmune diseases at the periphery. In order to determine whether this is also the case in the brain, brain and plasma concentrations of IL-1? and IL-1Ra were measured by ELISA in a model of chronic brain inflammation in Lewis rats, the hippocampal delayed-type hypersensitivity (DTH) response to bacillus Calmette–Guérin (BCG). Brain IL-1? increased rapidly after intracerebral (i.c.) injection of BCG and came back to baseline concentrations 1 week later, whereas IL-1Ra increased gradually over time and remained elevated during the last 2 weeks post-BCG intracerebral injection. Following peripheral BCG challenge, brain IL-1? increased at the site of the brain BCG and peaked 12 days later before decreasing on day 16 post-challenge. Brain IL-1Ra remained elevated during the first days post-challenge and then decreased from the 12th day post-challenge. The same temporal variations were observed in the plasma concentrations of IL-1? and IL-1Ra. The increase in the IL-1?/IL-1Ra ratio that was apparent from day 3 to day 12 post-challenge might be correlated with the invasion of peripheral inflammatory cells at the site of intracerebral injection. Besides showing that the course of inflammation alters the brain IL-1?/IL-1Ra ratio, these findings point to the importance of monitoring plasma IL-1?/IL-1Ra ratio to predict the course of brain inflammation.

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Published date: April 2004

Identifiers

Local EPrints ID: 56576
URI: http://eprints.soton.ac.uk/id/eprint/56576
ISSN: 0165-5728
PURE UUID: 4a821f4a-ea7a-412a-942f-c134682e1001

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Date deposited: 08 Aug 2008
Last modified: 08 Jan 2022 19:04

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Contributors

Author: Karine Palin
Author: Danièle Verrier
Author: Viviane Tridon
Author: Jane Hurst
Author: V. Hugh Perry
Author: Robert Dantzer
Author: Jacques Lestage

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