The University of Southampton
University of Southampton Institutional Repository

Survival of Listeria monocytogenes Scott a on metal surfaces: Implications for cross-contamination

Wilks, S.A., MichelS, H.T. and Keevil, C.W. (2006) Survival of Listeria monocytogenes Scott a on metal surfaces: Implications for cross-contamination International Journal of Food Microbiology, 111, (2), pp. 93-98. (doi:10.1016/j.ijfoodmicro.2006.04.037).

Record type: Article


Listeria monocytogenes is an important re-emerging pathogen which is commonly found in the environment. Many outbreaks have been associated with the contamination of food produce, often linked to cross-contamination from surfaces or equipment to prepared foodstuffs. In the present study a number of copper-base metal alloys have been used to assess the survival times of L. monocytogenes on different materials, in comparison with stainless steel. High concentrations (107) of bacteria were placed on metal coupons cut from each alloy. After defined incubation times, coupons were placed in tubes containing phosphate buffered saline and vortexed to remove the cells. Aliquots were then plated onto tryptone blood agar plates and the number of colony forming units counted. The high concentration of bacteria was used to represent a “worst-case” scenario. The results indicate that survival is greatly reduced on a copper-base alloy compared to stainless steel. Viable cells could be detected on stainless steel after 24 h incubation at room temperature. On copper, brass, aluminium bronze and silicon bronze, no viable bacteria could be detected after 60 min incubation, indicating a 5 log reduction (the detection limit of the procedure was 100 bacteria). No cells could be detected from copper nickel and copper nickel zinc alloys, after 90 min incubation. The viability stain, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), confirmed these results, with actively respiring bacteria being clearly labelled on stainless steel after 24 h. The results suggest that careful choice of surface material could reduce the potential risk of cross-contamination in industrial, commercial and domestic environments.

Full text not available from this repository.

More information

Submitted date: 28 April 2005
Published date: 1 September 2006
Keywords: listeria monocytogenes, copper alloys, survival, cross-contamination


Local EPrints ID: 56595
ISSN: 0168-1605
PURE UUID: 3668355f-5a59-4f54-8051-763530d8fad5
ORCID for S.A. Wilks: ORCID iD
ORCID for C.W. Keevil: ORCID iD

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record



Author: S.A. Wilks ORCID iD
Author: H.T. MichelS
Author: C.W. Keevil ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.