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Mannose receptor expression specifically reveals perivascular macrophages in normal, injured, and diseased mouse brain

Mannose receptor expression specifically reveals perivascular macrophages in normal, injured, and diseased mouse brain
Mannose receptor expression specifically reveals perivascular macrophages in normal, injured, and diseased mouse brain
Perivascular macrophages are believed to have a significant role in inflammation in the central nervous system (CNS). They express a number of different receptors that point toward functions in both innate immunity, through pathogen-associated molecular pattern recognition, phagocytosis, and cytokine responsiveness, and acquired immunity, through antigen presentation and co-stimulation. We are interested in the receptors that are differentially expressed by perivascular macrophages and microglia in both the normal CNS as well as in neuroinflammation and neurodegeneration. In this article we report the use of a well-characterized monoclonal antibody, 5D3, to localize the expression of the mannose receptor to perivascular macrophages in the normal CNS and in various models of brain pathology. Mannose receptor expression was limited to perivascular, meningeal, and choroid plexus macrophages in normal, inflamed, injured, and diseased CNS. In particular, activated microglia and invading hematogenous leukocytes were mannose receptor negative while expressing the F4/80 antigen, macrosialin (CD68), FcRII (CD32), scavenger receptor (CD204), and CR3 (CD11b/CD18). Since the perivascular macrophages expressing the mannose receptor are known to be the only constitutively phagocytic cells in the normal CNS, we injected clodronate-loaded liposomes intracerebroventricularly in control mice to deplete these cells. In these mice, there was no detectable mannose receptor expression in perivascular spaces after immunocytochemistry with the 5D3 monoclonal antibody. This finding underlines the value of the monoclonal antibody 5D3 as a tool to study murine perivascular macrophages selectively. Mannose receptor expression by macrophages located at blood-brain (perivascular), brain-cerebrospinal fluid (CSF) (meningeal), and CSF-blood (choroid plexus) interfaces supports a functional role of these cells in responding to external stimuli such as infection.
brain, perivascular, macrophage, mannose receptor, mouse, immunocytochemistry, clodronate, liposomes
0894-1491
375-384
Galea, Ian
66209a2f-f7e6-4d63-afe4-e9299f156f0b
Palin, Karine
395fbcda-515f-4e3f-aac6-1c292d114a89
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Van Rooijen, Nico
a9f3fc94-192d-4e2d-b662-765bd778bc55
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Boche, Delphine
bdcca10e-6302-4dd0-919f-67218f7e0d61
Galea, Ian
66209a2f-f7e6-4d63-afe4-e9299f156f0b
Palin, Karine
395fbcda-515f-4e3f-aac6-1c292d114a89
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Van Rooijen, Nico
a9f3fc94-192d-4e2d-b662-765bd778bc55
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Boche, Delphine
bdcca10e-6302-4dd0-919f-67218f7e0d61

Galea, Ian, Palin, Karine, Newman, Tracey A., Van Rooijen, Nico, Perry, V. Hugh and Boche, Delphine (2005) Mannose receptor expression specifically reveals perivascular macrophages in normal, injured, and diseased mouse brain. GLIA, 49 (3), 375-384. (doi:10.1002/glia.20124).

Record type: Article

Abstract

Perivascular macrophages are believed to have a significant role in inflammation in the central nervous system (CNS). They express a number of different receptors that point toward functions in both innate immunity, through pathogen-associated molecular pattern recognition, phagocytosis, and cytokine responsiveness, and acquired immunity, through antigen presentation and co-stimulation. We are interested in the receptors that are differentially expressed by perivascular macrophages and microglia in both the normal CNS as well as in neuroinflammation and neurodegeneration. In this article we report the use of a well-characterized monoclonal antibody, 5D3, to localize the expression of the mannose receptor to perivascular macrophages in the normal CNS and in various models of brain pathology. Mannose receptor expression was limited to perivascular, meningeal, and choroid plexus macrophages in normal, inflamed, injured, and diseased CNS. In particular, activated microglia and invading hematogenous leukocytes were mannose receptor negative while expressing the F4/80 antigen, macrosialin (CD68), FcRII (CD32), scavenger receptor (CD204), and CR3 (CD11b/CD18). Since the perivascular macrophages expressing the mannose receptor are known to be the only constitutively phagocytic cells in the normal CNS, we injected clodronate-loaded liposomes intracerebroventricularly in control mice to deplete these cells. In these mice, there was no detectable mannose receptor expression in perivascular spaces after immunocytochemistry with the 5D3 monoclonal antibody. This finding underlines the value of the monoclonal antibody 5D3 as a tool to study murine perivascular macrophages selectively. Mannose receptor expression by macrophages located at blood-brain (perivascular), brain-cerebrospinal fluid (CSF) (meningeal), and CSF-blood (choroid plexus) interfaces supports a functional role of these cells in responding to external stimuli such as infection.

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Submitted date: 3 April 2004
Published date: 1 February 2005
Keywords: brain, perivascular, macrophage, mannose receptor, mouse, immunocytochemistry, clodronate, liposomes

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Local EPrints ID: 56647
URI: https://eprints.soton.ac.uk/id/eprint/56647
ISSN: 0894-1491
PURE UUID: bfe5a669-962d-4f6d-85f1-31f789d2c42a
ORCID for Ian Galea: ORCID iD orcid.org/0000-0002-1268-5102
ORCID for Tracey A. Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for Delphine Boche: ORCID iD orcid.org/0000-0002-5884-130X

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Date deposited: 11 Aug 2008
Last modified: 14 Mar 2019 01:51

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Contributors

Author: Ian Galea ORCID iD
Author: Karine Palin
Author: Nico Van Rooijen
Author: V. Hugh Perry
Author: Delphine Boche ORCID iD

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