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Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods

Lidwell, K., Dillon, J., Sihota, A., Connor, V. and Pilkington, B. (2004) Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods Biochemical Society Transactions, 32, (5), pp. 868-870.

Record type: Article

Abstract

mGluRs (metabotropic glutamate receptors) are G-protein-coupled receptors that modulate synaptic transmission. The eight mammalian mGluRs form three groups based on sequence and functional similarities: group I (1 and 5), group II (2 and 3) and group III (4, 6–8) mGluRs. In the present study, we used a Y2H (yeast two hybrid) screen to identify proteins that interact with the C-terminal intracellular tail of mGluR3. Prominent among the candidate receptor interacting proteins was calmodulin, a Ca2+ sensor known to bind identifiable sequences in group I and III mGluRs. The Y2H method was used to investigate calmodulin binding to mGluRs but failed to confirm the documented interaction with group III mGluRs. Furthermore, subsequent biochemical analysis showed that calmodulin does not interact with group II mGluRs. This illustrates that certain Ca2+-dependent interactions are not recapitulated in yeast. Moreover, it highlights the necessity for supporting biochemical data to substantiate interactions identified with Y2H methods.

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More information

Published date: 1 November 2004
Keywords: calmodulin, metabotropic glutamate receptor (mGluR), protein interaction, yeast two-hybrid (Y2H) screen

Identifiers

Local EPrints ID: 56683
URI: http://eprints.soton.ac.uk/id/eprint/56683
ISSN: 0300-5127
PURE UUID: 88e4a00f-332d-439b-8868-3b711e641d49

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Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: K. Lidwell
Author: J. Dillon
Author: A. Sihota
Author: V. Connor
Author: B. Pilkington

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