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Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods

Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods
Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods
We have established a transcription map of individual bovine embryos using semiquantitative reverse transcriptase-polymerase chain reaction to detect the levels of six marker genes involved in early embryo differentiation. The critical step of compaction during preimplantation development is often not accomplished or it takes place for only a short period in in vitro generated embryos, which may result in reduced viability. Compaction is accompanied by the assembly of intercellular tight junctions (TJs) as a barrier against the extraembryonic environment and as a prerequisite for blastocele formation. In the present study, we have related the expression of TJ gene mRNA in individual bovine embryos to their developmental stage, their competence to undergo a clear period of compaction before blastocyst formation, and their in vitro or in vivo origin. Our results indicate that embryos that showed a detectable and well-formed compaction period in vitro are of similar quality to their in vivo counterparts. Starting from the same amount of maternal message, in vivo and in vitro development differ most during the critical period of the major switch from maternal to embryonic genomic control before a dramatic increase of TJ mRNAs occurs upon blastocyst formation. Failure to compact in vitro results in significant reduction of specific transcript levels, in a manner that depends on culture conditions, which may contribute to reduced viability. We conclude that TJ mRNA expression levels are sensitive to environmental conditions that may influence the developmental potential of bovine blastocysts.
1394-1402
Miller, D.J.
528f92f1-9ec9-45f3-968f-b7a1f2714d08
Eckert, J.J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Lazzari, G.
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Duranthon-Richoux, V.
e7f49ef7-a379-47b8-a90b-9ac44391fc28
Sreenan, J.
e73d8e94-083b-4ef6-aaa3-8511e5a732ad
Morris, D.
f0bacd4d-81b7-44c1-917b-92aae1187e8b
Galli, C.
6823f562-790e-486e-afa3-ca23747ab71a
Renard, J.P.
6168053f-a485-4626-bb13-ba223ee2e02f
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Miller, D.J.
528f92f1-9ec9-45f3-968f-b7a1f2714d08
Eckert, J.J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Lazzari, G.
47b225ff-55da-408c-9b6e-910928bd2d4e
Duranthon-Richoux, V.
e7f49ef7-a379-47b8-a90b-9ac44391fc28
Sreenan, J.
e73d8e94-083b-4ef6-aaa3-8511e5a732ad
Morris, D.
f0bacd4d-81b7-44c1-917b-92aae1187e8b
Galli, C.
6823f562-790e-486e-afa3-ca23747ab71a
Renard, J.P.
6168053f-a485-4626-bb13-ba223ee2e02f
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03

Miller, D.J., Eckert, J.J., Lazzari, G., Duranthon-Richoux, V., Sreenan, J., Morris, D., Galli, C., Renard, J.P. and Fleming, T.P. (2003) Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods. Biology of Reproduction, 68, 1394-1402. (doi:10.1095/biolreprod.102.009951).

Record type: Article

Abstract

We have established a transcription map of individual bovine embryos using semiquantitative reverse transcriptase-polymerase chain reaction to detect the levels of six marker genes involved in early embryo differentiation. The critical step of compaction during preimplantation development is often not accomplished or it takes place for only a short period in in vitro generated embryos, which may result in reduced viability. Compaction is accompanied by the assembly of intercellular tight junctions (TJs) as a barrier against the extraembryonic environment and as a prerequisite for blastocele formation. In the present study, we have related the expression of TJ gene mRNA in individual bovine embryos to their developmental stage, their competence to undergo a clear period of compaction before blastocyst formation, and their in vitro or in vivo origin. Our results indicate that embryos that showed a detectable and well-formed compaction period in vitro are of similar quality to their in vivo counterparts. Starting from the same amount of maternal message, in vivo and in vitro development differ most during the critical period of the major switch from maternal to embryonic genomic control before a dramatic increase of TJ mRNAs occurs upon blastocyst formation. Failure to compact in vitro results in significant reduction of specific transcript levels, in a manner that depends on culture conditions, which may contribute to reduced viability. We conclude that TJ mRNA expression levels are sensitive to environmental conditions that may influence the developmental potential of bovine blastocysts.

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Published date: 1 April 2003

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Local EPrints ID: 56714
URI: http://eprints.soton.ac.uk/id/eprint/56714
PURE UUID: 69cbb54e-e408-4792-a994-bab57f7a92bf

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Date deposited: 07 Aug 2008
Last modified: 15 Mar 2024 11:03

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Contributors

Author: D.J. Miller
Author: J.J. Eckert
Author: G. Lazzari
Author: V. Duranthon-Richoux
Author: J. Sreenan
Author: D. Morris
Author: C. Galli
Author: J.P. Renard
Author: T.P. Fleming

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