The University of Southampton
University of Southampton Institutional Repository

Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease

Record type: Article

Background Our aim was to understand why some sera from patients with a broad spectrum of autoimmune diseases or non-autoimmune diseases involving enhanced apoptosis, cell lysis and/or putative secondary autoimmune processes show reactions in the tissue transglutaminase (TGc) ELISA used for diagnosis of gluten-sensitive disease.
Methods Sera were compared from groups of patients with autoimmune diseases, diseases involving organ specific enhanced cell death, celiac disease or dermatitis herpetiformis, diseases of non-autoimmune origin, and a group without known disease. IgA antibodies against TGc were detected using human antigen (produced recombinantly in bacterial or human cells) in different systems (non-commercial ELISA with buffers of differing NaCl concentrations, and anti-TGc sandwich ELISA). Anti-gliadin and anti-endomysium antibodies were also determined.
Results Many sera from patients with autoimmune disorders gave a positive signal in the human TGc ELISAs. The signal appeared related to minor impurities in the recombinant human TGc used and to raised serum IgA antibody levels rather than to the occurrence of TGc specific antibodies in these patients.
Conclusions No association of anti-TGc Abs and autoimmune conditions independent of gluten-sensitive disease could be shown. Care should be taken to exclude copurification of chaperones, like heat shock protein 70, where preparing antigens for TGc ELISAs.
Keywords: Tissue transglutaminase; Celiac disease; Gluten-sensitive disease; Autoimmunity; ELISA

Full text not available from this repository.

Citation

Sardy, M., Csikos, M., Geisen, C., Preisz, K., Kornsee, Z., Tomsits, E., Tox, U., Hunzelmann, N., Wieslander, J., Karpati, S., Paulsson, M. and Smyth, N. (2007) Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease Clinica Chimica Acta, 376, (1-2), pp. 126-135. (doi:10.1016/j.cca.2006.08.006).

More information

Published date: 1 February 2007
Keywords: tissue transglutaminase, celiac disease, gluten-sensitive disease, autoimmunity, ELISA

Identifiers

Local EPrints ID: 56733
URI: http://eprints.soton.ac.uk/id/eprint/56733
ISSN: 0009-8981
PURE UUID: 62948022-a2c2-4bfe-b975-d8d40f14ab2f

Catalogue record

Date deposited: 08 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record

Altmetrics

Contributors

Author: M. Sardy
Author: M. Csikos
Author: C. Geisen
Author: K. Preisz
Author: Z. Kornsee
Author: E. Tomsits
Author: U. Tox
Author: N. Hunzelmann
Author: J. Wieslander
Author: S. Karpati
Author: M. Paulsson
Author: N. Smyth

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×