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Methods for imaging labeled neurons together with neuropil features in Drosophila

Record type: Article

We describe staining protocols for serial semithin sections of Drosophila central ganglia that allow visualization of gene expression in particular neurons with counterstaining to display the ganglion architecture. Green fluorescent protein (GFP), expressed in a subset of sensory neurons from a selected enhancer trap line, is visualized by conventional immunohistochemistry with a peroxidase-linked antibody, and neural architecture is revealed by reduced silver staining. This makes visible in histological sections the same GFP-labeled cells seen with confocal microscopy, but with the especial advantage that neuropil structures are also revealed at the level of individual cells and neuron processes. Not only does this allow the physical relationships among intracellularly labeled neurons to be determined by reference to specific features in the neuropil but it also enables a function to be ascribed provisionally to particular regions of neuropil. These methods have particular utility for mapping morphological information on specific neurons in the context of central nervous system architecture, both in adult Drosophila and during development.

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Citation

Tyrer, N.M., Shepherd, D. and Williams, D.W. (2000) Methods for imaging labeled neurons together with neuropil features in Drosophila Journal of Histochemistry and Cytochemistry, 48, (11), pp. 1575-1581.

More information

Published date: November 2000

Identifiers

Local EPrints ID: 56755
URI: http://eprints.soton.ac.uk/id/eprint/56755
ISSN: 0022-1554
PURE UUID: f03c08df-1492-4a20-9910-ef72f93ea3f2

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Date deposited: 22 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: N.M. Tyrer
Author: D. Shepherd
Author: D.W. Williams

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