The University of Southampton
University of Southampton Institutional Repository

Methods for imaging labeled neurons together with neuropil features in Drosophila

Tyrer, N.M., Shepherd, D. and Williams, D.W. (2000) Methods for imaging labeled neurons together with neuropil features in Drosophila Journal of Histochemistry and Cytochemistry, 48, (11), pp. 1575-1581.

Record type: Article


We describe staining protocols for serial semithin sections of Drosophila central ganglia that allow visualization of gene expression in particular neurons with counterstaining to display the ganglion architecture. Green fluorescent protein (GFP), expressed in a subset of sensory neurons from a selected enhancer trap line, is visualized by conventional immunohistochemistry with a peroxidase-linked antibody, and neural architecture is revealed by reduced silver staining. This makes visible in histological sections the same GFP-labeled cells seen with confocal microscopy, but with the especial advantage that neuropil structures are also revealed at the level of individual cells and neuron processes. Not only does this allow the physical relationships among intracellularly labeled neurons to be determined by reference to specific features in the neuropil but it also enables a function to be ascribed provisionally to particular regions of neuropil. These methods have particular utility for mapping morphological information on specific neurons in the context of central nervous system architecture, both in adult Drosophila and during development.

Full text not available from this repository.

More information

Published date: November 2000


Local EPrints ID: 56755
ISSN: 0022-1554
PURE UUID: f03c08df-1492-4a20-9910-ef72f93ea3f2

Catalogue record

Date deposited: 22 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record


Author: N.M. Tyrer
Author: D. Shepherd
Author: D.W. Williams

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.