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The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase

The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase
The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase
Mammalian c-Jun N-terminal kinases (JNKs) are members of a group of stress-activated intracellular signalling molecules within the MAP kinase family. Molecular genetic analysis of a highly evolutionarily conserved Drosophila JNK homologue, DJNK, has demonstrated that this molecule plays an essential developmental role in cell shape regulation. However, it remains to be determined whether DJNK also responds to the broad range of cellular stresses and other stimuli that affect its mammalian counterpart. Here we demonstrate that c-Jun, a substrate for mammalian JNKs, is a specific substrate for DJNK and that an antiserum that cross-reacts with activated mammalian JNK at the conserved threonyl-prolyl-tyrosyl (TPY) motif within the activation loop also specifically recognises the activated form of DJNK. Using these two assays, we show that DJNK activity is stimulated in cultured cells by several treatments that activate mammalian JNKs, including addition of arsenite, vanadate and ceramide derivatives. It is therefore concluded that in addition to its essential developmental functions, DJNK plays an important role in stress responses that mirrors its mammalian counterpart.
0965-1748
839-847
Botella, J.A.
9ffe4c76-7bab-40c5-ba9f-d69916f5f60e
Baines, I.A.
b3d04e85-768f-49c0-bd4d-23c78a9ab23c
Williams, D.D.
1428b355-db6e-4a92-9c49-38a888c8f9ef
Goberdhan, D.C.I.
bebd7dd5-ff65-4b7f-8fc4-4fc1594fca4d
Proud, C.G.
c2cc50f9-4565-4d59-9dfc-aa70b9268a6e
Wilson, C.
8f81973c-e436-4727-97fd-de70017a9f56
Botella, J.A.
9ffe4c76-7bab-40c5-ba9f-d69916f5f60e
Baines, I.A.
b3d04e85-768f-49c0-bd4d-23c78a9ab23c
Williams, D.D.
1428b355-db6e-4a92-9c49-38a888c8f9ef
Goberdhan, D.C.I.
bebd7dd5-ff65-4b7f-8fc4-4fc1594fca4d
Proud, C.G.
c2cc50f9-4565-4d59-9dfc-aa70b9268a6e
Wilson, C.
8f81973c-e436-4727-97fd-de70017a9f56

Botella, J.A., Baines, I.A., Williams, D.D., Goberdhan, D.C.I., Proud, C.G. and Wilson, C. (2001) The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase. Insect Biochemistry and Molecular Biology, 31 (9), 839-847. (doi:10.1016/S0965-1748(01)00029-7).

Record type: Article

Abstract

Mammalian c-Jun N-terminal kinases (JNKs) are members of a group of stress-activated intracellular signalling molecules within the MAP kinase family. Molecular genetic analysis of a highly evolutionarily conserved Drosophila JNK homologue, DJNK, has demonstrated that this molecule plays an essential developmental role in cell shape regulation. However, it remains to be determined whether DJNK also responds to the broad range of cellular stresses and other stimuli that affect its mammalian counterpart. Here we demonstrate that c-Jun, a substrate for mammalian JNKs, is a specific substrate for DJNK and that an antiserum that cross-reacts with activated mammalian JNK at the conserved threonyl-prolyl-tyrosyl (TPY) motif within the activation loop also specifically recognises the activated form of DJNK. Using these two assays, we show that DJNK activity is stimulated in cultured cells by several treatments that activate mammalian JNKs, including addition of arsenite, vanadate and ceramide derivatives. It is therefore concluded that in addition to its essential developmental functions, DJNK plays an important role in stress responses that mirrors its mammalian counterpart.

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Published date: 1 July 2001

Identifiers

Local EPrints ID: 56764
URI: https://eprints.soton.ac.uk/id/eprint/56764
ISSN: 0965-1748
PURE UUID: 9bf2de73-a9fb-41f6-9b77-03b3a31156fe

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Date deposited: 08 Aug 2008
Last modified: 13 Mar 2019 20:34

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Contributors

Author: J.A. Botella
Author: I.A. Baines
Author: D.D. Williams
Author: D.C.I. Goberdhan
Author: C.G. Proud
Author: C. Wilson

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