The University of Southampton
University of Southampton Institutional Repository

Stimulation of endocytosis in mouse blastocysts by insulin - a quantitative morphological analysis

Dunglison, G.F., Jane, S.D., McCaul, T.F., Chad, J.E., Fleming, T.P. and Kaye, P.L. (1995) Stimulation of endocytosis in mouse blastocysts by insulin - a quantitative morphological analysis Reproduction, 105, pp. 115-123. (doi:10.1530/jrf.0.1050115). (PMID:7490702).

Record type: Article

Abstract

The effects of insulin on the endocytic activity of mouse blastocysts in vitro were investigated using confocal laser scanning microscopy, quantitative image analysis and electron microscopy. Confocal studies showed that fluorescein isothiocyanate-labelled markers, dextran (fluid phase) and albumin (combined membrane and fluid phase), were endocytosed by blastocysts and localized within vesicles (about 2.5 ?m in diameter) in the outer trophectoderm cells. No labelling was detected in the inner cell mass cells or the blastocoel cavity. Treatment with 170 nmol insulin l?1 stimulated the endocytosis of fluorescently labelled dextran in freshly collected blastocysts, increasing mean vesicle diameter per embryo by 15% (P < 0.05) after incubation with insulin for 2.5 h and mean vesicle number per embryo by 56% (P < 0.01) after 6 h. Both effects were also evident in blastocysts that had been cultured from the late eight-cell stage. Blastocysts incubated for 6 h with insulin displayed increased convolutions in the trophectoderm apical membrane compared with controls, indicating increased membrane activity and suggesting macropinosome formation. Collectively, these results suggest that insulin enhances endocytosis in the trophectoderm by stimulating uptake at the apical membrane into larger and more numerous endocytic vesicles and with some evidence of vesicle fusion. This mechanism may provide a metabolic basis for the stimulation by insulin of biosynthesis, proliferation and morphological development in early embryos.

Full text not available from this repository.

More information

Published date: 1 September 1995

Identifiers

Local EPrints ID: 56826
URI: http://eprints.soton.ac.uk/id/eprint/56826
ISSN: 0022-4251
PURE UUID: badf3891-8acd-4f69-8c1c-798cdf3919ad
ORCID for J.E. Chad: ORCID iD orcid.org/0000-0001-6442-4281

Catalogue record

Date deposited: 26 Aug 2008
Last modified: 17 Jul 2017 14:30

Export record

Altmetrics

Contributors

Author: G.F. Dunglison
Author: S.D. Jane
Author: T.F. McCaul
Author: J.E. Chad ORCID iD
Author: T.P. Fleming
Author: P.L. Kaye

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×