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Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward

Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward
Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward
This study reports the isolation and characterization of seven highly polymorphic microsatellite loci in Chthamalus montagui (Crustacea, Cirripedia). The loci were isolated from a library constructed from genomic DNA enriched for CA repeats. The markers yielded three to 43 alleles per locus (mean 16.7) in samples averaging 49 individuals. Observed heterozygosity ranged from 0.08 to 0.58 (mean 0.39). These microsatellite loci will be valuable tools for population genetic studies of this species.
barnacle, Chthamalus montaguim, microsatellites, pcr
1471-8278
641-643
Pannacciulli, F.G.
0af40454-ffbf-41b7-9396-e7c6f21b7e4b
Piyapattanakorn, S.
703eb51a-7e23-461a-b1fd-093f04384dac
Bishop, J.D.D.
a9433edb-02a2-46a3-a75d-c5787b752597
Hawkins, S.J.
758fe1c1-30cd-4ed1-bb65-2471dc7c11fa
Maclean, N.
fc7272ea-27ce-4f19-bb5b-dbccb8c520a8
Pannacciulli, F.G.
0af40454-ffbf-41b7-9396-e7c6f21b7e4b
Piyapattanakorn, S.
703eb51a-7e23-461a-b1fd-093f04384dac
Bishop, J.D.D.
a9433edb-02a2-46a3-a75d-c5787b752597
Hawkins, S.J.
758fe1c1-30cd-4ed1-bb65-2471dc7c11fa
Maclean, N.
fc7272ea-27ce-4f19-bb5b-dbccb8c520a8

Pannacciulli, F.G., Piyapattanakorn, S., Bishop, J.D.D., Hawkins, S.J. and Maclean, N. (2005) Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward. Molecular Ecology Notes, 5 (3), 641-643. (doi:10.1111/j.1471-8286.2005.01027.x).

Record type: Article

Abstract

This study reports the isolation and characterization of seven highly polymorphic microsatellite loci in Chthamalus montagui (Crustacea, Cirripedia). The loci were isolated from a library constructed from genomic DNA enriched for CA repeats. The markers yielded three to 43 alleles per locus (mean 16.7) in samples averaging 49 individuals. Observed heterozygosity ranged from 0.08 to 0.58 (mean 0.39). These microsatellite loci will be valuable tools for population genetic studies of this species.

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Published date: 20 January 2005
Keywords: barnacle, Chthamalus montaguim, microsatellites, pcr
Organisations: Ocean and Earth Science
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Identifiers

Local EPrints ID: 56854
URI: http://eprints.soton.ac.uk/id/eprint/56854
DOI: doi:10.1111/j.1471-8286.2005.01027.x
ISSN: 1471-8278
PURE UUID: cc6bc118-ece8-4ba6-9acf-0b3cf3533ba0

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 06 Oct 2020 23:46

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Contributors

Author: F.G. Pannacciulli
Author: S. Piyapattanakorn
Author: J.D.D. Bishop
Author: S.J. Hawkins
Author: N. Maclean

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