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Protein patterning on silicon (100)

Johnson, C.W.A., Yin, H., Gore, M.G. and Melvin, T. (2005) Protein patterning on silicon (100) At BioScience 2005. 01 Jul 2005.

Record type: Conference or Workshop Item (Paper)

Abstract

Silicon is an ideal substrate for the development of micro and nano bio-molecular sensor devices; the fabrication technology developed for microelectronic industries is now finding extensive applications in the creation of microfluidic devices. To realise these devices an approach for the creation of precise, highly stable motifs of biomolecules on the silicon surface is needed. Covalent attachment of proteins to crystalline silicon (100) surfaces has recently been achieved in patterns with micron sized features, using a straightforward two-step process, by us. UV light exposure through a mask was used to covalently attach a monolayer of alkene, functionalized with N-Hydroxysuccinimide ester groups, onto a hydrogen-terminated silicon (100) surface. The N-Hydroxysuccinimide ester surface is a template for the subsequent bioconjugation of a single lg-binding domaion of Protein L via the protein amino group present in external lysine residues of the protein. The patterned surfaces were revealed using epifluorescence microscopy; fluorescently labelled Mouse lgG was associated with the surface attached protein L domain. This method is to be developed for the creation of novel microfluidic sensing technology in silicon for proteomic applications.

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More information

Published date: 2005
Venue - Dates: BioScience 2005, 2005-07-01 - 2005-07-01

Identifiers

Local EPrints ID: 57739
URI: http://eprints.soton.ac.uk/id/eprint/57739
PURE UUID: a8d64554-a797-48d1-88a4-9684c05e133e

Catalogue record

Date deposited: 11 Aug 2008
Last modified: 17 Jul 2017 14:28

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Contributors

Author: C.W.A. Johnson
Author: H. Yin
Author: M.G. Gore
Author: T. Melvin

University divisions

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