The University of Southampton
University of Southampton Institutional Repository

A green fluorescent protein with photoswitchable emission from the deep sea

A green fluorescent protein with photoswitchable emission from the deep sea
A green fluorescent protein with photoswitchable emission from the deep sea
A colorful variety of fluorescent proteins (FPs) from marine invertebrates are utilized as genetically encoded markers for live cell imaging. The increased demand for advanced imaging techniques drives a continuous search for FPs with new and improved properties. Many useful FPs have been isolated from species adapted to sun-flooded habitats such as tropical coral reefs. It has yet remained unknown if species expressing green fluorescent protein (GFP)-like proteins also exist in the darkness of the deep sea. Using a submarine-based and -operated fluorescence detection system in the Gulf of Mexico, we discovered ceriantharians emitting bright green fluorescence in depths between 500 and 600 m and identified a GFP, named cerFP505, with bright fluorescence emission peaking at 505 nm. Spectroscopic studies showed that ~15% of the protein bulk feature reversible ON/OFF photoswitching that can be induced by alternating irradiation with blue und near-UV light. Despite being derived from an animal adapted to essentially complete darkness and low temperatures, cerFP505 maturation in living mammalian cells at 37°C, its brightness and photostability are comparable to those of EGFP and cmFP512 from shallow water species. Therefore, our findings disclose the deep sea as a potential source of GFP-like molecular marker proteins.
1932-6203
e3766
Vogt, Alexander
3de2d0d9-468c-46ac-8f6c-721a9b1bd7a8
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Denzel, Andrea
41266ff8-cbf4-4195-96ab-07824ec50838
Mazel, Charles H.
24a058f4-c5c6-423f-8067-ea2f919629de
Matz, Mikhail V.
9a128396-eaf1-4c49-aac4-aa160d01e393
Invanchenko, Sergey
009027b3-d7d7-4771-82d7-33f7038c62bc
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Vogt, Alexander
3de2d0d9-468c-46ac-8f6c-721a9b1bd7a8
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Denzel, Andrea
41266ff8-cbf4-4195-96ab-07824ec50838
Mazel, Charles H.
24a058f4-c5c6-423f-8067-ea2f919629de
Matz, Mikhail V.
9a128396-eaf1-4c49-aac4-aa160d01e393
Invanchenko, Sergey
009027b3-d7d7-4771-82d7-33f7038c62bc
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7

Vogt, Alexander, D'Angelo, Cecilia, Oswald, Franz, Denzel, Andrea, Mazel, Charles H., Matz, Mikhail V., Invanchenko, Sergey, Nienhaus, G. Ulrich and Wiedenmann, Jörg (2008) A green fluorescent protein with photoswitchable emission from the deep sea. PLoS ONE, 3 (11), e3766. (doi:10.1371/journal.pone.0003766).

Record type: Article

Abstract

A colorful variety of fluorescent proteins (FPs) from marine invertebrates are utilized as genetically encoded markers for live cell imaging. The increased demand for advanced imaging techniques drives a continuous search for FPs with new and improved properties. Many useful FPs have been isolated from species adapted to sun-flooded habitats such as tropical coral reefs. It has yet remained unknown if species expressing green fluorescent protein (GFP)-like proteins also exist in the darkness of the deep sea. Using a submarine-based and -operated fluorescence detection system in the Gulf of Mexico, we discovered ceriantharians emitting bright green fluorescence in depths between 500 and 600 m and identified a GFP, named cerFP505, with bright fluorescence emission peaking at 505 nm. Spectroscopic studies showed that ~15% of the protein bulk feature reversible ON/OFF photoswitching that can be induced by alternating irradiation with blue und near-UV light. Despite being derived from an animal adapted to essentially complete darkness and low temperatures, cerFP505 maturation in living mammalian cells at 37°C, its brightness and photostability are comparable to those of EGFP and cmFP512 from shallow water species. Therefore, our findings disclose the deep sea as a potential source of GFP-like molecular marker proteins.

Other
fetchObject.action_uri=info_doi%2F10.1371%2Fjournal.pone.0003766&representation=PDF - Version of Record
Restricted to Repository staff only
Available under License Other.
Request a copy

More information

Published date: 19 November 2008
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 58898
URI: http://eprints.soton.ac.uk/id/eprint/58898
ISSN: 1932-6203
PURE UUID: df17eb6c-e171-435a-bb56-e7bd982ac62e
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 19 Aug 2008
Last modified: 17 Dec 2019 01:44

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×