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Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders

Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders
Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders
In myeloproliferative disorders (MPDs), basophils typically increase in number in the bone marrow (BM) and blood. In chronic myeloid leukemia (CML), basophilia is a diagnostic and prognostic variable. However, no reliable approach for routine detection and enumeration of basophils in BM sections is available. We applied the antibasogranulin antibody BB1 on paraffin-embedded BM sections in 21 control samples (normal BM), 45 patients with CML, 9 with chronic idiopathic myelofibrosis, 11 with polycythemia vera, 19 with essential thrombocythemia, and 7 with indolent systemic mastocytosis. As assessed by immunostaining of serial BM sections, BB1+ cells coexpressed myeloperoxidase, histidine decarboxylase, and leukosialin but did not express B- or T-cell-restricted antigens. BB1+ BM cells were found to be highly elevated in patients with CML compared with normal BM or other MPDs, with maximum counts found in accelerated phase CML (median, 160 cells/mm(2)). In summary, BB1 (basogranulin) is a new immunohistochemical basophil marker that should allow quantification of basophils in CML at diagnosis and during therapy.
myeloproliferative disorders, chronic myeloid leukemia, cml, basophils, basogranulin
0002-9173
273-281
Agis, Hermine
9a354161-039c-40ec-a98d-b8745c26e824
Krauth, Maria-Theresa
8840a17d-82ac-4151-8633-8b82f37c2b63
Böhm, Alexandra
20d16f5a-7d21-48ef-ba12-7c2f81f5edfe
Mosberger, Isabella
783a91b2-f53c-4269-851e-01ba2aca450f
Müllauer, Leonhard
02795d56-9f7b-4c82-997d-958c4702cc2f
Simonitsch-Klupp, Ingrid
7bf34e9f-c3ab-4dd0-aec7-beb12f3f4775
Walls, Andrew F.
c7f79f37-0b21-43f6-a32e-8256664d7f90
Horny, Hans-Peter
95077a3b-b869-49ba-a227-f88b2c0bad80
Valent, Peter
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Agis, Hermine
9a354161-039c-40ec-a98d-b8745c26e824
Krauth, Maria-Theresa
8840a17d-82ac-4151-8633-8b82f37c2b63
Böhm, Alexandra
20d16f5a-7d21-48ef-ba12-7c2f81f5edfe
Mosberger, Isabella
783a91b2-f53c-4269-851e-01ba2aca450f
Müllauer, Leonhard
02795d56-9f7b-4c82-997d-958c4702cc2f
Simonitsch-Klupp, Ingrid
7bf34e9f-c3ab-4dd0-aec7-beb12f3f4775
Walls, Andrew F.
c7f79f37-0b21-43f6-a32e-8256664d7f90
Horny, Hans-Peter
95077a3b-b869-49ba-a227-f88b2c0bad80
Valent, Peter
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe

Agis, Hermine, Krauth, Maria-Theresa, Böhm, Alexandra, Mosberger, Isabella, Müllauer, Leonhard, Simonitsch-Klupp, Ingrid, Walls, Andrew F., Horny, Hans-Peter and Valent, Peter (2006) Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders. American Journal of Clinical Pathology, 125 (2), 273-281. (doi:10.1309/M9FQMQGF66167N2X).

Record type: Article

Abstract

In myeloproliferative disorders (MPDs), basophils typically increase in number in the bone marrow (BM) and blood. In chronic myeloid leukemia (CML), basophilia is a diagnostic and prognostic variable. However, no reliable approach for routine detection and enumeration of basophils in BM sections is available. We applied the antibasogranulin antibody BB1 on paraffin-embedded BM sections in 21 control samples (normal BM), 45 patients with CML, 9 with chronic idiopathic myelofibrosis, 11 with polycythemia vera, 19 with essential thrombocythemia, and 7 with indolent systemic mastocytosis. As assessed by immunostaining of serial BM sections, BB1+ cells coexpressed myeloperoxidase, histidine decarboxylase, and leukosialin but did not express B- or T-cell-restricted antigens. BB1+ BM cells were found to be highly elevated in patients with CML compared with normal BM or other MPDs, with maximum counts found in accelerated phase CML (median, 160 cells/mm(2)). In summary, BB1 (basogranulin) is a new immunohistochemical basophil marker that should allow quantification of basophils in CML at diagnosis and during therapy.

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Published date: February 2006
Keywords: myeloproliferative disorders, chronic myeloid leukemia, cml, basophils, basogranulin

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Local EPrints ID: 59259
URI: http://eprints.soton.ac.uk/id/eprint/59259
ISSN: 0002-9173
PURE UUID: 9f81df07-4986-47a1-801c-ace0affe8118

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Date deposited: 02 Sep 2008
Last modified: 13 Mar 2019 20:30

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Author: Hermine Agis
Author: Maria-Theresa Krauth
Author: Alexandra Böhm
Author: Isabella Mosberger
Author: Leonhard Müllauer
Author: Ingrid Simonitsch-Klupp
Author: Andrew F. Walls
Author: Hans-Peter Horny
Author: Peter Valent

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