The University of Southampton
University of Southampton Institutional Repository

Human keratinocyte induction of rapid effector function in antigen-specific memory CD4+ and CD8+ T cells

Human keratinocyte induction of rapid effector function in antigen-specific memory CD4+ and CD8+ T cells
Human keratinocyte induction of rapid effector function in antigen-specific memory CD4+ and CD8+ T cells
The ability of human keratinocytes to present antigen to T cells is controversial and, indeed, it has been suggested that keratinocytes may promote T cell hyporesponsiveness. Furthermore, it is unclear whether keratinocytes can process antigen prior to MHC class I and class II presentation. We tested the ability of keratinocytes to induce functional responses in epitope-specific CD4+ and CD8+ memory T cells using peptides, protein and recombinant expression vectors as sources of antigen. Keratinocytes were able to efficiently process and present protein antigen to CD4+ T cells, resulting in cytokine secretion (Th1 and Th2). This interaction was dependent on keratinocyte expression of HLA class II and ICAM-1, which could be induced by IFN-gamma. In addition, keratinocytes could present virally encoded or exogenous peptide to CD8+ T cells, resulting in T cell cytokine production and target cell lysis. Finally, T cell lines grown using keratinocytes as stimulators showed no loss of function. These findings demonstrate that keratinocytes are able to efficiently process and present antigen to CD4+ and CD8+ memory T cells and induce functional responses. The findings have broad implications for the pathogenesis of cutaneous disease and for transcutaneous drug or vaccine delivery.
antigen presentation, keratinocytes, skin
0014-2980
1485-1493
Black, Antony P.B.
24caf91a-4616-4cae-b7fa-ea3283f1bfc9
Ardern-Jones, Michael R.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Kasprowicz, Victoria
28cebde0-dda6-4433-ba45-c3039b1223a7
Bowness, Paul
b8c5ab84-2528-4cd2-b44e-f1eb3a45baa9
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Bailey, Abigail S.
4c25f1b0-554b-45f0-92ad-8a5d79bb9fe5
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358
Black, Antony P.B.
24caf91a-4616-4cae-b7fa-ea3283f1bfc9
Ardern-Jones, Michael R.
7ac43c24-94ab-4d19-ba69-afaa546bec90
Kasprowicz, Victoria
28cebde0-dda6-4433-ba45-c3039b1223a7
Bowness, Paul
b8c5ab84-2528-4cd2-b44e-f1eb3a45baa9
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Bailey, Abigail S.
4c25f1b0-554b-45f0-92ad-8a5d79bb9fe5
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358

Black, Antony P.B., Ardern-Jones, Michael R., Kasprowicz, Victoria, Bowness, Paul, Jones, Louise, Bailey, Abigail S. and Ogg, Graham S. (2007) Human keratinocyte induction of rapid effector function in antigen-specific memory CD4+ and CD8+ T cells. European Journal of Immunology, 37 (6), 1485-1493. (doi:10.1002/eji.200636915).

Record type: Article

Abstract

The ability of human keratinocytes to present antigen to T cells is controversial and, indeed, it has been suggested that keratinocytes may promote T cell hyporesponsiveness. Furthermore, it is unclear whether keratinocytes can process antigen prior to MHC class I and class II presentation. We tested the ability of keratinocytes to induce functional responses in epitope-specific CD4+ and CD8+ memory T cells using peptides, protein and recombinant expression vectors as sources of antigen. Keratinocytes were able to efficiently process and present protein antigen to CD4+ T cells, resulting in cytokine secretion (Th1 and Th2). This interaction was dependent on keratinocyte expression of HLA class II and ICAM-1, which could be induced by IFN-gamma. In addition, keratinocytes could present virally encoded or exogenous peptide to CD8+ T cells, resulting in T cell cytokine production and target cell lysis. Finally, T cell lines grown using keratinocytes as stimulators showed no loss of function. These findings demonstrate that keratinocytes are able to efficiently process and present antigen to CD4+ and CD8+ memory T cells and induce functional responses. The findings have broad implications for the pathogenesis of cutaneous disease and for transcutaneous drug or vaccine delivery.

Full text not available from this repository.

More information

Published date: June 2007
Keywords: antigen presentation, keratinocytes, skin

Identifiers

Local EPrints ID: 59270
URI: https://eprints.soton.ac.uk/id/eprint/59270
ISSN: 0014-2980
PURE UUID: 717ee38a-5648-4fec-9b51-fd05a321839d

Catalogue record

Date deposited: 02 Sep 2008
Last modified: 13 Mar 2019 20:30

Export record

Altmetrics

Contributors

Author: Antony P.B. Black
Author: Victoria Kasprowicz
Author: Paul Bowness
Author: Louise Jones
Author: Abigail S. Bailey
Author: Graham S. Ogg

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×