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Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release.

Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release.
Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release.
Dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. This technique was used to evaluate the effect of cyclosporin A, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. Four beagle dogs spontaneously sensitized to Ascaris suum were treated for 1 month with oral cyclosporin A. At days 0, 15 and 30 of the treatment, dialysis probes were inserted into the skin of the back, and 20 microL of A. suum antigen was injected intradermally at each site. At timed intervals, dialysate was collected and assayed for histamine and prostaglandin D(2) and the wheal area was measured. Mean histamine concentration and wheal area were significantly lower at days 15 and 30 of treatment, compared with day 0. However, prostaglandin D(2) concentration was not significantly reduced. The inhibition in histamine release after intradermal challenge, by cyclosporin, confirms its anti-inflammatory action in the dog. Dermal microdialysis provides a useful tool for investigating canine allergic reactions and their modulation by drugs.
0959-4493
169-174
Brazís, Pilar
c7da1a7a-1237-4c23-93ff-8874e4918423
Barandica, Liliana
3601c83b-04b7-4ee7-911b-d7d7b3fd2bf2
García, Félix
a48757e1-56fb-477f-a28c-0ac649bd857c
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Puigdemont, Anna
bfd4d98a-632d-445b-a2c6-2ed0aa61e318
Brazís, Pilar
c7da1a7a-1237-4c23-93ff-8874e4918423
Barandica, Liliana
3601c83b-04b7-4ee7-911b-d7d7b3fd2bf2
García, Félix
a48757e1-56fb-477f-a28c-0ac649bd857c
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Puigdemont, Anna
bfd4d98a-632d-445b-a2c6-2ed0aa61e318

Brazís, Pilar, Barandica, Liliana, García, Félix, Clough, Geraldine F., Church, Martin K. and Puigdemont, Anna (2006) Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release. Veterinary Dermatology, 17 (3), 169-174. (doi:10.1111/j.1365-3164.2006.00511.x).

Record type: Article

Abstract

Dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. This technique was used to evaluate the effect of cyclosporin A, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. Four beagle dogs spontaneously sensitized to Ascaris suum were treated for 1 month with oral cyclosporin A. At days 0, 15 and 30 of the treatment, dialysis probes were inserted into the skin of the back, and 20 microL of A. suum antigen was injected intradermally at each site. At timed intervals, dialysate was collected and assayed for histamine and prostaglandin D(2) and the wheal area was measured. Mean histamine concentration and wheal area were significantly lower at days 15 and 30 of treatment, compared with day 0. However, prostaglandin D(2) concentration was not significantly reduced. The inhibition in histamine release after intradermal challenge, by cyclosporin, confirms its anti-inflammatory action in the dog. Dermal microdialysis provides a useful tool for investigating canine allergic reactions and their modulation by drugs.

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Published date: 4 May 2006

Identifiers

Local EPrints ID: 59273
URI: https://eprints.soton.ac.uk/id/eprint/59273
ISSN: 0959-4493
PURE UUID: dcefcccf-6b57-42da-b153-16a086259288
ORCID for Geraldine F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 02 Sep 2008
Last modified: 14 Mar 2019 01:51

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Contributors

Author: Pilar Brazís
Author: Liliana Barandica
Author: Félix García
Author: Martin K. Church
Author: Anna Puigdemont

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