The University of Southampton
University of Southampton Institutional Repository

What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo?

What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo?
What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo?
This study examined the suitability of microdialysis to assess the time course of cytokine generation from discrete sites within the skin following intradermal injection of allergen. Cytokines were recovered using two microdialysis probes, one close to the point of allergen injection and the other 1 cm away but within the area of the late-phase induration. Skin biopsies taken at both sites were stained immunocytochemically to investigate possible relationships between cytokine generation, expression of adhesion molecules, and recruitment of neutrophils and eosinophils during the late-phase allergic response. The cytokine response to probe insertion was assessed using a single probe in the opposite arm (control). At baseline, microdialysate contained low levels of IL-1alpha, IL-5, IL-8, IL-12, GM-CSF, and TNFalpha (n=27-33). At control sites, this was followed by increases in IL-6 and IL-8 at 3 and 6 hours. Allergen increased TNFalpha levels in 3/11 individuals within 30 minutes at the injection site. Levels of IL-6 and IL-8 rose rapidly and were significantly greater (P<0.05) than that of controls at 3 and 6 hours at both injection and distant sites. Adhesion molecule expression and leukocyte infiltration were elevated only at the allergen injection site, suggesting a complex relationship between cytokine generation and cellular events in allergic inflammation. In conclusion, microdialysis can be used to distinguish temporal and spatial changes in protein profiles in the skin. Furthermore, when used in conjunction with skin biopsies, it provides novel information about the mechanisms of dermal inflammation.
0022-202X
2799-2806
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Jackson, Claire L.
ca0c242e-3638-4949-a0cb-f41e36067b8f
Lee, Jacob J.P.
bcafc141-2641-4da0-b347-06667a070088
Jamal, Sarah C.
32ae84db-15f2-469a-b64d-f135596ce1dc
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8
Clough, Geraldine F.
9f19639e-a929-4976-ac35-259f9011c494
Jackson, Claire L.
ca0c242e-3638-4949-a0cb-f41e36067b8f
Lee, Jacob J.P.
bcafc141-2641-4da0-b347-06667a070088
Jamal, Sarah C.
32ae84db-15f2-469a-b64d-f135596ce1dc
Church, Martin K.
dad189d5-866e-4ae1-b005-0d87f74282b8

Clough, Geraldine F., Jackson, Claire L., Lee, Jacob J.P., Jamal, Sarah C. and Church, Martin K. (2007) What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo? Journal of Investigative Dermatology, 127 (12), 2799-2806. (doi:10.1038/sj.jid.5700930).

Record type: Article

Abstract

This study examined the suitability of microdialysis to assess the time course of cytokine generation from discrete sites within the skin following intradermal injection of allergen. Cytokines were recovered using two microdialysis probes, one close to the point of allergen injection and the other 1 cm away but within the area of the late-phase induration. Skin biopsies taken at both sites were stained immunocytochemically to investigate possible relationships between cytokine generation, expression of adhesion molecules, and recruitment of neutrophils and eosinophils during the late-phase allergic response. The cytokine response to probe insertion was assessed using a single probe in the opposite arm (control). At baseline, microdialysate contained low levels of IL-1alpha, IL-5, IL-8, IL-12, GM-CSF, and TNFalpha (n=27-33). At control sites, this was followed by increases in IL-6 and IL-8 at 3 and 6 hours. Allergen increased TNFalpha levels in 3/11 individuals within 30 minutes at the injection site. Levels of IL-6 and IL-8 rose rapidly and were significantly greater (P<0.05) than that of controls at 3 and 6 hours at both injection and distant sites. Adhesion molecule expression and leukocyte infiltration were elevated only at the allergen injection site, suggesting a complex relationship between cytokine generation and cellular events in allergic inflammation. In conclusion, microdialysis can be used to distinguish temporal and spatial changes in protein profiles in the skin. Furthermore, when used in conjunction with skin biopsies, it provides novel information about the mechanisms of dermal inflammation.

Full text not available from this repository.

More information

Published date: 28 June 2007

Identifiers

Local EPrints ID: 59283
URI: https://eprints.soton.ac.uk/id/eprint/59283
ISSN: 0022-202X
PURE UUID: f664b1d9-891a-41d4-bf24-f473fd323438
ORCID for Geraldine F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

Catalogue record

Date deposited: 02 Sep 2008
Last modified: 20 Jul 2019 01:16

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×