The University of Southampton
University of Southampton Institutional Repository

Pregnancy induces numerical and functional changes of CD4+CD25high regulatory T cells in patients with rheumatoid arthritis

Förger, F., Marcoli, N., Gadola, S., Möller, B., Villiger, P.M. and Østensen, M. (2007) Pregnancy induces numerical and functional changes of CD4+CD25high regulatory T cells in patients with rheumatoid arthritis Annals of the Rheumatic Diseases, 67, (7), pp. 984-990. (doi:10.1136/ard.2007.075283).

Record type: Article

Abstract

OBJECTIVE: In a prospective study we investigated whether numerical and functional changes of CD4+CD25(high) regulatory T cells (Treg) were associated with changes of disease activity observed during pregnancy and post partum in patients with rheumatoid arthritis (RA). METHODS: The frequency of CD4+CD25(high) T cells was determined by flow cytometry in 12 patients with RA and 14 healthy women during and after pregnancy. FACS-sorted CD4+CD25(high) T cells and CD4+CD25- T cells were stimulated with anti-CD3 and anti-CD28 monoclonal antibodies alone or in co-culture to investigate proliferation and cytokine secretion. RESULTS: Frequencies of CD4+CD25(high) Treg were significantly higher in the third trimester compared to 8 weeks post-partum both in patients and controls. Numbers of CD4+CD25(high) Treg inversely correlated with disease activity in the third trimester and post-partum. In co-culture experiments significantly higher amounts of IL-10 and lowered levels of TNFalpha and IFNgamma were found in supernatants of the third trimester compared to post-partum samples. These findings were independent from health or disease in pregnancy, however post-partum TNFalpha and IFNgamma levels were higher in patients with disease flares. CONCLUSION: The amelioration of disease activity in the third trimester corresponded to the increased number of Treg that induced a pronounced anti-inflammatory cytokine milieu. The pregnancy related quantitative and qualitative changes of Treg suggest a beneficial effect of Treg on disease activity.

Full text not available from this repository.

More information

Published date: 30 October 2007

Identifiers

Local EPrints ID: 59297
URI: http://eprints.soton.ac.uk/id/eprint/59297
ISSN: 0003-4967
PURE UUID: 1a07e6cd-8cc3-441d-bb6c-85662e2e6e16

Catalogue record

Date deposited: 19 Jan 2009
Last modified: 17 Jul 2017 14:24

Export record

Altmetrics

Contributors

Author: F. Förger
Author: N. Marcoli
Author: S. Gadola
Author: B. Möller
Author: P.M. Villiger
Author: M. Østensen

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×