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The cysteinyl-leukotriene type 1 receptor polymorphism 927T/C is associated with atopy severity but not with asthma

The cysteinyl-leukotriene type 1 receptor polymorphism 927T/C is associated with atopy severity but not with asthma
The cysteinyl-leukotriene type 1 receptor polymorphism 927T/C is associated with atopy severity but not with asthma
BACKGROUND: The cysteinyl-leukotriene receptor type 1 (CysLT1) mediates the bronchoconstrictor and pro-inflammatory actions of cysteinyl-leukotrienes (LTC4, LTD4, LTE4) in asthma and is the molecular target of the lukast class of oral anti-leukotriene drugs. We screened the CYSLTR1 gene on chromosome Xq13-21 for coding region polymorphisms, and investigated their associations with allergy and asthma.
METHODS: Solid-phase chemical cleavage was used to screen polymorphisms in the coding region of CYSLTR1. A TaqMan allelic discrimination assay was used to genotype a 927T/C SNP and oligonucleotide ligation assays were used to genotype the previously reported 617T/C and 898G/A SNPs of CYSLTR1 in 341 asthmatic families from the UK. Associations with asthma diagnosis, atopic status, serum-specific IgE and severity of allergy and asthma were examined.
RESULTS: Family-based association tests showed that the 927 T allele was associated with atopy severity, especially in female subjects, but not with asthma diagnosis or severity, atopic status, bronchial hyper-responsiveness to methacholine or forced expiratory volume in 1 s.
CONCLUSION: Mutation screening identified only one polymorphism, 927T/C, in the coding region of the CysLT1 receptor. This polymorphsim is predictive of atopy severity, but not associated with asthma.
asthma, atopy, genetic, leukotriene receptor, polymorphism
0954-7894
735-741
Hao, L.
7617ddd7-90e9-44b2-af12-717babd9c0ee
Sayers, I.
230e01df-0685-438a-9173-df1aead72390
Cakebread, J.A.
5af7ac0c-2782-49b5-a1df-5e3051e5ea33
Barton, S.J.
4f674382-ca0b-44ad-9670-e71a0b134ef0
Beghé, B.
c20830d1-e2ba-4ddd-af37-7c49dc82311a
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Hao, L.
7617ddd7-90e9-44b2-af12-717babd9c0ee
Sayers, I.
230e01df-0685-438a-9173-df1aead72390
Cakebread, J.A.
5af7ac0c-2782-49b5-a1df-5e3051e5ea33
Barton, S.J.
4f674382-ca0b-44ad-9670-e71a0b134ef0
Beghé, B.
c20830d1-e2ba-4ddd-af37-7c49dc82311a
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Sampson, A.P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a

Hao, L., Sayers, I., Cakebread, J.A., Barton, S.J., Beghé, B., Holgate, S.T., Sampson, A.P. and Holloway, J.W. (2006) The cysteinyl-leukotriene type 1 receptor polymorphism 927T/C is associated with atopy severity but not with asthma. Clinical & Experimental Allergy, 36 (6), 735-741. (doi:10.1111/j.1365-2222.2006.02511.x).

Record type: Article

Abstract

BACKGROUND: The cysteinyl-leukotriene receptor type 1 (CysLT1) mediates the bronchoconstrictor and pro-inflammatory actions of cysteinyl-leukotrienes (LTC4, LTD4, LTE4) in asthma and is the molecular target of the lukast class of oral anti-leukotriene drugs. We screened the CYSLTR1 gene on chromosome Xq13-21 for coding region polymorphisms, and investigated their associations with allergy and asthma.
METHODS: Solid-phase chemical cleavage was used to screen polymorphisms in the coding region of CYSLTR1. A TaqMan allelic discrimination assay was used to genotype a 927T/C SNP and oligonucleotide ligation assays were used to genotype the previously reported 617T/C and 898G/A SNPs of CYSLTR1 in 341 asthmatic families from the UK. Associations with asthma diagnosis, atopic status, serum-specific IgE and severity of allergy and asthma were examined.
RESULTS: Family-based association tests showed that the 927 T allele was associated with atopy severity, especially in female subjects, but not with asthma diagnosis or severity, atopic status, bronchial hyper-responsiveness to methacholine or forced expiratory volume in 1 s.
CONCLUSION: Mutation screening identified only one polymorphism, 927T/C, in the coding region of the CysLT1 receptor. This polymorphsim is predictive of atopy severity, but not associated with asthma.

Full text not available from this repository.

More information

Published date: 1 June 2006
Keywords: asthma, atopy, genetic, leukotriene receptor, polymorphism

Identifiers

Local EPrints ID: 59308
URI: http://eprints.soton.ac.uk/id/eprint/59308
ISSN: 0954-7894
PURE UUID: 784c3d6c-d82d-4dbb-8e88-c44922e5787d
ORCID for S.J. Barton: ORCID iD orcid.org/0000-0003-4963-4242
ORCID for J.W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 02 Sep 2008
Last modified: 05 Nov 2019 01:59

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