Association of TNF-alpha genetic polymorphism with HLA DPB1*0301

Kim, S.H., Ye, Y.M., Lee, S.K., Choi, J.H., Holloway, J.W., Park, C.S. and Park, H.S. (2006) Association of TNF-alpha genetic polymorphism with HLA DPB1*0301. Clinical & Experimental Allergy, 36 (10), 1247-1253. (doi:10.1111/j.1365-2222.2006.02567.x).

Abstract

BACKGROUND: We speculated TNF-alpha can be one of candidate gene for aspirin-intolerant asthma (AIA) because TNF-alpha is pro-inflammatory cytokine and known to be increased level in asthmatic airways. In addition, genetic interaction between TNF-alpha and human antigen leucocyte (HLA) DPB1*0301, which is a strong genetic marker for AIA, was examined for its close location within chromosome 6.
METHOD: To investigate genetic association of TNF-alpha with an AIA phenotype, three study groups (163 patients with AIA, 197 patients with aspirin-tolerant asthma (ATA), 257 normal control subjects) were enrolled. Single nucleotide polymorphisms (SNPs) were genotyped using a single-base extension method and HLA DPB1 genotyping was determined by high-throughput sequencing method.
RESULTS: All five SNPs of TNF-alpha were tested; there were no significant differences in allele and genotype frequencies among the three groups. However, significant association between TNF-alpha-308G>A polymorphism and atopy status was noted (PT, P<0.001, respectively).
CONCLUSION: TNF-alpha promoter polymorphism may significantly increase susceptibility to AIA by gene-to-gene interaction with HLA DPB1*0301.

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Contributors

Author: J.W. Holloway

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