Cellular localization of interleukin 13 receptor alpha2 in human primary bronchial epithelial cells and fibroblasts
Cellular localization of interleukin 13 receptor alpha2 in human primary bronchial epithelial cells and fibroblasts
Background: Interleukin (IL) 13 is a key cytokine in asthma, regulating fibrosis, airway remodeling, induction of immunoglobulin E synthesisby B cells, bronchial hyperresponsiveness, and mucus production. IL-13 signals through the type II IL-4 receptor (IL-4R), which is composed of the IL-4R? and the IL-13R?1 chains. Another IL-13 binding chain, IL-13R?2, binds IL-13 with high affinity but has no known signaling capability and is thought to serve as a decoy receptor providing tight regulation of IL-13 responses.
Methods: In this study, we investigated the cellular localization of IL-13R?2 in human primary bronchial epithelial cells and fibroblasts using flow cytometry and confocal microscopy, as well as the in vivo expression of IL-13R?2 in the human bronchial mucosa by means of immunohistochemistry.
Results: IL-13R?2 is predominantly an intracellular rather than a membrane-bound molecule in both human primary bronchial epithelial cells and fibroblasts and displays a diffuse granular cytoplasmic distribution in both cell types. IL-13R?2 protein is expressed in vivo in the human bronchial mucosa with its expression being higher in bronchial epithelial cells than bronchial fibroblasts both in vivo and in vitro.
Conclusions: IL-13R?2 is expressed by both human primary bronchial epithelial cells and fibroblasts as an intracellular protein with a diffuse cytoplasmic distribution. In vivo, IL-13R?2 is expressed in the human airway mucosa mainly by bronchial epithelial cells.
IL-13 receptor alpha2, intracellular protein, cellular localization, cytoplasmic compartment, bronchial epithelial cells, bronchial fibroblasts
174-180
Konstantinidis, A.K.
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Puddicombe, S.M.
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Mochizuki, A.
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Sheth, P.D.
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Yang, I.A.
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Yoshisue, H.
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Wilson, S.J.
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Davies, D.E.
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Holgate, S.T.
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Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
2008
Konstantinidis, A.K.
46895f79-6ef0-4686-bb77-30a0b3a59ffc
Puddicombe, S.M.
124e2c4e-ab9a-46f3-855c-b54ed0b61cc4
Mochizuki, A.
a1c9d7a5-6597-4bfa-99aa-5e23b576a5fa
Sheth, P.D.
e550ade9-e94b-46aa-87ce-7b62d896d6d7
Yang, I.A.
0da6e2cb-d200-440e-ba70-48fb8e7738cd
Yoshisue, H.
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Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Konstantinidis, A.K., Puddicombe, S.M., Mochizuki, A., Sheth, P.D., Yang, I.A., Yoshisue, H., Wilson, S.J., Davies, D.E., Holgate, S.T. and Holloway, J.W.
(2008)
Cellular localization of interleukin 13 receptor alpha2 in human primary bronchial epithelial cells and fibroblasts.
Journal of Investigational Allergology and Clinical Immunology, 18 (3), .
(PMID:18564628)
Abstract
Background: Interleukin (IL) 13 is a key cytokine in asthma, regulating fibrosis, airway remodeling, induction of immunoglobulin E synthesisby B cells, bronchial hyperresponsiveness, and mucus production. IL-13 signals through the type II IL-4 receptor (IL-4R), which is composed of the IL-4R? and the IL-13R?1 chains. Another IL-13 binding chain, IL-13R?2, binds IL-13 with high affinity but has no known signaling capability and is thought to serve as a decoy receptor providing tight regulation of IL-13 responses.
Methods: In this study, we investigated the cellular localization of IL-13R?2 in human primary bronchial epithelial cells and fibroblasts using flow cytometry and confocal microscopy, as well as the in vivo expression of IL-13R?2 in the human bronchial mucosa by means of immunohistochemistry.
Results: IL-13R?2 is predominantly an intracellular rather than a membrane-bound molecule in both human primary bronchial epithelial cells and fibroblasts and displays a diffuse granular cytoplasmic distribution in both cell types. IL-13R?2 protein is expressed in vivo in the human bronchial mucosa with its expression being higher in bronchial epithelial cells than bronchial fibroblasts both in vivo and in vitro.
Conclusions: IL-13R?2 is expressed by both human primary bronchial epithelial cells and fibroblasts as an intracellular protein with a diffuse cytoplasmic distribution. In vivo, IL-13R?2 is expressed in the human airway mucosa mainly by bronchial epithelial cells.
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Published date: 2008
Keywords:
IL-13 receptor alpha2, intracellular protein, cellular localization, cytoplasmic compartment, bronchial epithelial cells, bronchial fibroblasts
Organisations:
Faculty of Medicine, Human Genetics, Infection Inflammation & Immunity
Identifiers
Local EPrints ID: 59357
URI: http://eprints.soton.ac.uk/id/eprint/59357
ISSN: 1018-9068
PURE UUID: 3d2d5b94-6e4c-4e35-a21f-26d5c9daec14
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Date deposited: 11 Sep 2008
Last modified: 16 Mar 2024 02:57
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Author:
A.K. Konstantinidis
Author:
S.M. Puddicombe
Author:
A. Mochizuki
Author:
P.D. Sheth
Author:
I.A. Yang
Author:
H. Yoshisue
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