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Tissue localization of human trefoil factors 1, 2, and 3

Madsen, Jens, Nielsen, Ole, Tornøe, Ida, Thim, Lars and Holmskov, Uffe (2007) Tissue localization of human trefoil factors 1, 2, and 3 Journal of Histochemistry and Cytochemistry, 55, (5), pp. 505-513. (doi:10.1369/jhc.6A7100.2007).

Record type: Article

Abstract

Trefoil factors (TTFs) are small, compact proteins coexpressed with mucins in the gastrointestinal tract. Three trefoil factors are known in mammals: TFF1, TFF2, and TFF3. They are implicated to play diverse roles in maintenance and repair of the gastrointestinal channel. We compared the expression pattern of the three trefoil factors analyzing mRNA from a panel of 20 human tissues by conventional reverse transcriptase (RT) PCR and, in addition, by real-time PCR. These findings were supported by immunohistochemical analysis of paraffin-embedded human tissues using rabbit polyclonal antibodies raised against these factors. TFF1 showed highest expression in the stomach and colon, whereas TFF2 and TFF3 showed highest expression in stomach and colon, respectively. All three TFFs were found in the ducts of pancreas. Whereas TFF2 was found to be restricted to these two tissues, the structurally more closely related TFF1 and TFF3 showed a more general tissue distribution and were found to colocalize on an array of mucosal surfaces. This is the first thorough parallel description of the tissue distribution of TFFs in normal tissues, and it provides a baseline for similar analysis in diseased tissues.

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More information

Published date: May 2007
Keywords: trefoil factor, mucosal, innate immunity, tissue localization, real-time pcr, immunohistochemistry

Identifiers

Local EPrints ID: 59372
URI: http://eprints.soton.ac.uk/id/eprint/59372
ISSN: 0022-1554
PURE UUID: 3b71f1e3-6c6f-42d2-b31c-ff98525a8f7b
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645

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Date deposited: 03 Sep 2008
Last modified: 17 Jul 2017 14:24

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Contributors

Author: Jens Madsen ORCID iD
Author: Ole Nielsen
Author: Ida Tornøe
Author: Lars Thim
Author: Uffe Holmskov

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