The University of Southampton
University of Southampton Institutional Repository

Behind the chlamydial cloak: the replication cycle of chlamydiaphage Chp2, revealed

Record type: Article

Studying the replication of the chlamydiaphages presents significant challenges. Their host bacteria, chlamydiae, have a unique obligate intracellular developmental cycle. Using qPCR, immunochemistry, and electron microscopy, the life cycle of chlamydiaphage Chp2 was characterised. Chp2 infection has a dramatic inhibitory effect on bacterial cell division. The RB to EB transition is arrested and RBs enlarge without further division. There is a phase of rapid Chp2 genome replication 36 to 48 h post infection that is coincident with the expression of viral proteins and the replication of the host chromosome. The end stage of Chp2 replication is characterised by the appearance of paracrystalline structures followed by bacterial cell lysis. These data indicate that the Chp2 life cycle is closely coordinated with the developmental cycle of its bacterial host. This is a remarkable adaptation by a microvirus to infect and replicate in a bacterial host that has an obligate intracellular developmental cycle.

Full text not available from this repository.

Citation

Salim, Omar, Skilton, Rachel J., Lambden, Paul R., Fane, Bentley A. and Clarke, Ian N. (2008) Behind the chlamydial cloak: the replication cycle of chlamydiaphage Chp2, revealed Journal of Virology, 377, (2), pp. 440-445. (doi:10.1016/j.virol.2008.05.001).

More information

Published date: August 2008
Keywords: chlamydiaphage, Chp2, chlamydia, developmental cycle, life cycle

Identifiers

Local EPrints ID: 59406
URI: http://eprints.soton.ac.uk/id/eprint/59406
ISSN: 0022-538X
PURE UUID: 18fd5bb1-2b18-41c4-812b-fdf859a81dda
ORCID for Omar Salim: ORCID iD orcid.org/0000-0002-2562-4827

Catalogue record

Date deposited: 04 Sep 2008
Last modified: 17 Jul 2017 14:24

Export record

Altmetrics

Contributors

Author: Omar Salim ORCID iD
Author: Rachel J. Skilton
Author: Paul R. Lambden
Author: Bentley A. Fane
Author: Ian N. Clarke

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×