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Physicochemical characteristics and biological activities of seasonal atmospheric particulate matter sampling in two locations of Paris

Physicochemical characteristics and biological activities of seasonal atmospheric particulate matter sampling in two locations of Paris
Physicochemical characteristics and biological activities of seasonal atmospheric particulate matter sampling in two locations of Paris
Fine particulate matter present in urban areas seems to be incriminated in respiratory disorders. The aim of this study was to relate physicochemical characteristics of PM2.5 (particulate matter collected with a 50% efficiency for particles with an aerodynamic diameter of 2.5 µm) to their biological activities toward a bronchial epithelial cell line 16-HBE. Two seasonal sampling campaigns of particles were realized, respectively, in a kerbside and an urban background station in Paris. Sampled-PM2.5 mainly consist of particles with a size below 1 µm and are mainly composed of soot as assessed by analytical scanning electron microscopy. The different PM2.5 samples contrasted in their PAH content, which was the highest in the kerbside station in winter, as well as in their metal content. Kerbside station samples were characterized by the highest Fe and Cu content, which appears correlated to their hydroxyl radical generating properties measured by electron paramagnetic resonance. Particles were compared by their capacity to induce cytotoxicity, intracellular ROS production, and proinflammatory cytokine release (GM-CSF and TNF-alpha). At a concentration of 10 µg/cm2, all samples induced peroxide production and cytokine release to the similar extent in the absence of cytotoxicity. In conclusion, whereas the PM2.5 samples differ by their PAH and metal composition, they induce the same biological responses likely either due to components bioavailability and/ or interactions between PM components.
cytology, seasons, air pollutants, chemistry, respiratory tract diseases, research, drug effects, cytokines, reactive oxygen species, paris, chemically induced, urban health, cell line, particle size, peroxides, microscopy, activity, france, toxicity, analysis, humans, vehicle emissions, epithelial cells, metabolism, research support, oxygen, cell division, bronchi
0013-936X
5985-5992
Baulig, A.
f42ae13b-604e-433a-83cf-448a71a6e33a
Poirault, J.J.
2a1bcc2e-2fd2-4285-81bb-61ff56cdd31d
Ausset, P.
74459320-a270-4cf1-9d42-d926e55e751d
Schins, R.
8edecf75-88c7-4ea3-b2d2-8bd781333e9a
Shi, T.
9b811a34-4038-4463-b48c-e08b82e705b2
Baralle, D.
faac16e5-7928-4801-9811-8b3a9ea4bb91
Dorlhene, P.
4781dae4-b323-4821-b34d-a9ec71bf20d4
Meyer, M.
25c726d1-8f52-4a77-b913-ae79d4930cd1
Lefevre, R.
6cbfcd70-36b7-462a-b96c-135ba44bdb16
Baeza-Squiban, A.
1bd6258d-066f-406f-a1f4-a5835a682e8e
Marano, F.
6e256890-9f4e-4f0e-8322-02b60482059e
Baulig, A.
f42ae13b-604e-433a-83cf-448a71a6e33a
Poirault, J.J.
2a1bcc2e-2fd2-4285-81bb-61ff56cdd31d
Ausset, P.
74459320-a270-4cf1-9d42-d926e55e751d
Schins, R.
8edecf75-88c7-4ea3-b2d2-8bd781333e9a
Shi, T.
9b811a34-4038-4463-b48c-e08b82e705b2
Baralle, D.
faac16e5-7928-4801-9811-8b3a9ea4bb91
Dorlhene, P.
4781dae4-b323-4821-b34d-a9ec71bf20d4
Meyer, M.
25c726d1-8f52-4a77-b913-ae79d4930cd1
Lefevre, R.
6cbfcd70-36b7-462a-b96c-135ba44bdb16
Baeza-Squiban, A.
1bd6258d-066f-406f-a1f4-a5835a682e8e
Marano, F.
6e256890-9f4e-4f0e-8322-02b60482059e

Baulig, A., Poirault, J.J., Ausset, P., Schins, R., Shi, T., Baralle, D., Dorlhene, P., Meyer, M., Lefevre, R., Baeza-Squiban, A. and Marano, F. (2004) Physicochemical characteristics and biological activities of seasonal atmospheric particulate matter sampling in two locations of Paris. Environmental Science & Technology, 38 (22), 5985-5992. (doi:10.1021/es049476z).

Record type: Article

Abstract

Fine particulate matter present in urban areas seems to be incriminated in respiratory disorders. The aim of this study was to relate physicochemical characteristics of PM2.5 (particulate matter collected with a 50% efficiency for particles with an aerodynamic diameter of 2.5 µm) to their biological activities toward a bronchial epithelial cell line 16-HBE. Two seasonal sampling campaigns of particles were realized, respectively, in a kerbside and an urban background station in Paris. Sampled-PM2.5 mainly consist of particles with a size below 1 µm and are mainly composed of soot as assessed by analytical scanning electron microscopy. The different PM2.5 samples contrasted in their PAH content, which was the highest in the kerbside station in winter, as well as in their metal content. Kerbside station samples were characterized by the highest Fe and Cu content, which appears correlated to their hydroxyl radical generating properties measured by electron paramagnetic resonance. Particles were compared by their capacity to induce cytotoxicity, intracellular ROS production, and proinflammatory cytokine release (GM-CSF and TNF-alpha). At a concentration of 10 µg/cm2, all samples induced peroxide production and cytokine release to the similar extent in the absence of cytotoxicity. In conclusion, whereas the PM2.5 samples differ by their PAH and metal composition, they induce the same biological responses likely either due to components bioavailability and/ or interactions between PM components.

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More information

Published date: November 2004
Keywords: cytology, seasons, air pollutants, chemistry, respiratory tract diseases, research, drug effects, cytokines, reactive oxygen species, paris, chemically induced, urban health, cell line, particle size, peroxides, microscopy, activity, france, toxicity, analysis, humans, vehicle emissions, epithelial cells, metabolism, research support, oxygen, cell division, bronchi

Identifiers

Local EPrints ID: 59494
URI: http://eprints.soton.ac.uk/id/eprint/59494
ISSN: 0013-936X
PURE UUID: 9b4b3679-e405-4330-ae59-d11a986f6d75
ORCID for D. Baralle: ORCID iD orcid.org/0000-0003-3217-4833

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Date deposited: 04 Sep 2008
Last modified: 19 Mar 2021 02:40

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Contributors

Author: A. Baulig
Author: J.J. Poirault
Author: P. Ausset
Author: R. Schins
Author: T. Shi
Author: D. Baralle ORCID iD
Author: P. Dorlhene
Author: M. Meyer
Author: R. Lefevre
Author: A. Baeza-Squiban
Author: F. Marano

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