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Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology

Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology
Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology
Oligodendrocytes form an insulating multilamellar structure of compact myelin around axons, thereby allowing rapid propagation of action potentials. Despite the considerable clinical importance of myelination, little is known about the molecular mechanisms that enable oligodendrocytes to generate their specialized membrane wrapping. Here, we used microarray expression profiling of oligodendrocyte-ablated mutant mice to identify new glial molecules that are involved in CNS myelination. This effort resulted in the identification of Ermin, a novel cytoskeletal molecule that is exclusively expressed by oligodendrocytes. Ermin appears at a late stage during myelination, and in the mature nerves, it is localized to the outer cytoplasmic lip of the myelin sheath and the paranodal loops. In cultured oligodendrocytes, Ermin becomes visible in well differentiated MBP-positive cells, where it is concentrated at the tip of F-actin-rich processes (termed "Ermin spikes"). Ectopic expression of Ermin, but not of a mutant protein lacking its actin-binding domain, induced the formation of numerous cell protrusions and a pronounced change in cell morphology. Our results demonstrate that Ermin is a novel marker of myelinating oligodendroglia and suggest that it plays a role in cytoskeletal rearrangements during the late wrapping and/or compaction phases of myelinogenesis.
rats, cytoskeletal proteins, genetics, comparative study, neurologic mutants, axons, research, metabolism, proteins, protein, oligodendroglia, action potentials, research support, expression, biology, biosynthesis, mice, physiology, animals, transgenic, cultured, cells, cytology, role, myelin proteins
0270-6474
757-762
Brockschnieder, D.
766bdfda-70f4-4c9d-82e4-96ab38497161
Sabanay, H.
070fecb7-e1ac-4bf9-9e4a-25414e12416a
Riethmacher, D.
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Peles, E.
7b585b9c-54a6-4121-8ff5-f7a21b1e8c49
Brockschnieder, D.
766bdfda-70f4-4c9d-82e4-96ab38497161
Sabanay, H.
070fecb7-e1ac-4bf9-9e4a-25414e12416a
Riethmacher, D.
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Peles, E.
7b585b9c-54a6-4121-8ff5-f7a21b1e8c49

Brockschnieder, D., Sabanay, H., Riethmacher, D. and Peles, E. (2006) Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology. Journal of Neuroscience, 26 (3), 757-762. (doi:10.1523/JNEUROSCI.4317-05.2006).

Record type: Article

Abstract

Oligodendrocytes form an insulating multilamellar structure of compact myelin around axons, thereby allowing rapid propagation of action potentials. Despite the considerable clinical importance of myelination, little is known about the molecular mechanisms that enable oligodendrocytes to generate their specialized membrane wrapping. Here, we used microarray expression profiling of oligodendrocyte-ablated mutant mice to identify new glial molecules that are involved in CNS myelination. This effort resulted in the identification of Ermin, a novel cytoskeletal molecule that is exclusively expressed by oligodendrocytes. Ermin appears at a late stage during myelination, and in the mature nerves, it is localized to the outer cytoplasmic lip of the myelin sheath and the paranodal loops. In cultured oligodendrocytes, Ermin becomes visible in well differentiated MBP-positive cells, where it is concentrated at the tip of F-actin-rich processes (termed "Ermin spikes"). Ectopic expression of Ermin, but not of a mutant protein lacking its actin-binding domain, induced the formation of numerous cell protrusions and a pronounced change in cell morphology. Our results demonstrate that Ermin is a novel marker of myelinating oligodendroglia and suggest that it plays a role in cytoskeletal rearrangements during the late wrapping and/or compaction phases of myelinogenesis.

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More information

Published date: January 2006
Keywords: rats, cytoskeletal proteins, genetics, comparative study, neurologic mutants, axons, research, metabolism, proteins, protein, oligodendroglia, action potentials, research support, expression, biology, biosynthesis, mice, physiology, animals, transgenic, cultured, cells, cytology, role, myelin proteins

Identifiers

Local EPrints ID: 59526
URI: https://eprints.soton.ac.uk/id/eprint/59526
ISSN: 0270-6474
PURE UUID: bce087ea-9d56-43ff-9537-bcf1a9f611c7

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Date deposited: 03 Sep 2008
Last modified: 13 Mar 2019 20:30

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