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Allelic imbalance on chromosomes 13 and 17 and mutation analysis of BRCA1 and BRCA2 genes in monozygotic twins concordant for breast cancer

Record type: Article

To study genetic changes associated with the development of breast cancer and the extent of its hereditary predisposition, paraffin-embedded tissue samples were obtained from monozygotic twin pairs concordant for breast cancer through the linked Swedish Twin and Cancer Registries. DNA samples extracted from the matched tumour and normal tissues of nine twin pairs were analysed for allelic imbalance using a series of microsatellite markers on chromosomes 13 and 17, containing loci with known tumour suppressor genes. Multiple losses of constitutional heterozygosity (LOH), consistent with a loss of large genomic region, the whole chromosome or chromosome arm, was found in at least three pairs of twins. One double mitotic crossover was identified in one tumour sample in a pair concordant for LOH at multiple loci on both chromosomes. Recombination breakpoints were mapped to regions delineated by D13S218 and D13S263, and D13S155 and D13S279, respectively. In general, no genetic effect of losing the same allele within a twin pair was found. However, for one marker at chromosome 13 (D13S328, between the BRCA2 and the RB-1 loci) and two markers on chromosome 17 (D17S786, distal to the p53 locus, and D17S855, an intragenic BRCA1 marker) the proportion of twin pairs with the same LOH was significantly higher than expected. These regions may reflect hereditary genomic changes in our sample set. In addition, tumour DNA samples from a subset of 12 twin pairs were analysed for BRCA1 and BRCA2 mutations using exon-by-exon single-strand conformation polymorphism analysis. Two unclassified BRCA2 variants, with a putative pathogenic effect, were identified, but no pathogenic alterations were found in the BRCA1 gene.

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Citation

Forsti, Asta, Luo, Liping, Vorechovsky, Igor, Soderberg, Magnus, Lichtenstein, Paul and Hemminki, Kari (2001) Allelic imbalance on chromosomes 13 and 17 and mutation analysis of BRCA1 and BRCA2 genes in monozygotic twins concordant for breast cancer Carcinogenesis, 22, (1), pp. 27-33. (doi:10.1093/carcin/22.1.27).

More information

Published date: January 2001
Keywords: pair 13, female, monozygotic, chromosomes, chromosome mapping, diseases in twins, humans, neoplasm proteins, genes, non-u.s.gov't, binomial distribution, environmental, adult, arm, genetics, pair 17, breast cancer, loss of heterozygosity, dna mutational analysis, human, middle aged, brca1, protein, proteins, sweden, pathology, twins, transcription factors, dna, aged, mutation, cancer, breast neoplasms, research support, analysis, registries, brca2 protein

Identifiers

Local EPrints ID: 59731
URI: http://eprints.soton.ac.uk/id/eprint/59731
ISSN: 0143-3334
PURE UUID: 34ee5daf-8883-4222-8988-892571d402c9

Catalogue record

Date deposited: 04 Sep 2008
Last modified: 17 Jul 2017 14:24

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Contributors

Author: Asta Forsti
Author: Liping Luo
Author: Magnus Soderberg
Author: Paul Lichtenstein
Author: Kari Hemminki

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