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Differences in matrix metalloproteinase-1 and matrix metalloproteinase-12 transcript levels among carotid atherosclerotic plaques with different histopathological characteristics

Morgan, Angharad R., Rerkasem, Kittipan, Gallagher, Patrick J., Zhang, Baiping, Morris, Gareth E., Calder, Philip C., Grimble, Robert F., Eriksson, Per, McPheat, William L., Shearman, Clifford P. and Ye, Shu (2004) Differences in matrix metalloproteinase-1 and matrix metalloproteinase-12 transcript levels among carotid atherosclerotic plaques with different histopathological characteristics Stroke, 35, (6), pp. 1310-1315. (doi:10.1161/01.STR.0000126822.01756.99).

Record type: Article

Abstract

Background and Purpose— Previous studies have shown that atherosclerotic lesions express a number of matrix metalloproteinases (MMPs). Here we investigated whether transcript levels of MMP-1, -3, -7, -9, and -12 in carotid atherosclerotic plaques were correlated with histological features and clinical manifestations.
Methods— Atherosclerotic plaques (n=50) removed from patients undergoing carotid endarterectomy were classified histologically using a system proposed by Virmani et al, and MMP-1, -3, -7, -9, and -12 transcript levels in these tissues were quantified by real-time reverse-transcriptase polymerase chain reaction.
Results— Compared to plaques with a thick fibrous cap, those with a thin cap had a 7.8-fold higher MMP-1 transcript level (P=0.006). MMP-3, -7, and -12 were 1.5-fold, 1.8-fold, and 2.1-fold, respectively, higher in thin cap plaques, but the differences did not reach statistical significance. MMP-12 transcript levels were significantly increased in ruptured plaques compared with lesions without cap disruption (P=0.001). MMP-9 transcript levels were similar among the different types of lesion. MMP-1 and -12 transcript levels were significantly higher in plaques from patients with amaurosis fugax, than in those from asymptomatic patients (P=0.029 and P=0.008 for MMP-1 and MMP-12, respectively), than in those from patients with stroke (P=0.027 and P=0.001, respectively), and than in those from patients with transient ischemic attack (P=0.046 and P=0.008, respectively).
Conclusions— These data support a role of MMP-1 and -12 in determining atherosclerotic plaque stability.

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More information

Published date: 8 April 2004
Keywords: aged, role, reverse transcriptase polymerase chain reaction, human, female, metabolism, diagnosis, endarterectomy, male, rna, patients, research support, non-u.s.gov't, genetics, biosynthesis

Identifiers

Local EPrints ID: 60070
URI: http://eprints.soton.ac.uk/id/eprint/60070
ISSN: 0039-2499
PURE UUID: dda375c1-b45a-42a4-8881-ac1055364b53

Catalogue record

Date deposited: 08 Sep 2008
Last modified: 17 Jul 2017 14:23

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Contributors

Author: Angharad R. Morgan
Author: Kittipan Rerkasem
Author: Patrick J. Gallagher
Author: Baiping Zhang
Author: Gareth E. Morris
Author: Robert F. Grimble
Author: Per Eriksson
Author: William L. McPheat
Author: Shu Ye

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