The University of Southampton
University of Southampton Institutional Repository

Efficient transfer of HSV-1 amplicon vectors into embryonic stem cells and their derivatives

Efficient transfer of HSV-1 amplicon vectors into embryonic stem cells and their derivatives
Efficient transfer of HSV-1 amplicon vectors into embryonic stem cells and their derivatives
The derivation of specialized differentiated cells from embryonic stem (ES) cells is now a major focus for future therapies involving cell and organ replacement in humans. To obtain populations of differentiated cells for transplantation into the human body, highly optimized protocols are needed that allow direction of the development of ES cells and their derivatives. These protocols mostly include the use of a combination of growth factors that control the differentiation of ES cells into highly specialized cells found in adult organs. The introduction of different growth factors into ES cells and their derivatives via viral gene transfer may greatly facilitate the optimization of these protocols. In this chapter, we describe a method based on herpes simplex virus type 1, which allows efficient gene transfer during several steps of a protocol, directed to obtain neural progenitors from ES cells. This protocol therefore may allow the study of potential factors influencing the cell fate or differentiation of ES cells and their derivatives.
human, stem-cells, germany, adult, pluripotent stem cells, mice, cell differentiation, proteins, herpesvirus 1, population, neurons, growth, transplantation, therapy, protein, genetic vectors
1064-3745
265-272
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Lim, Filip
52e990b4-a6e7-4637-981c-6418bc381998
Schimmang, Thomas
a247597d-811d-495b-9b00-177a40a36477
Riethmacher, Dieter
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Lim, Filip
52e990b4-a6e7-4637-981c-6418bc381998
Schimmang, Thomas
a247597d-811d-495b-9b00-177a40a36477

Riethmacher, Dieter, Lim, Filip and Schimmang, Thomas (2006) Efficient transfer of HSV-1 amplicon vectors into embryonic stem cells and their derivatives. Methods in Molecular Biology, 329, 265-272. (doi:10.1385/1-59745-037-5:265).

Record type: Article

Abstract

The derivation of specialized differentiated cells from embryonic stem (ES) cells is now a major focus for future therapies involving cell and organ replacement in humans. To obtain populations of differentiated cells for transplantation into the human body, highly optimized protocols are needed that allow direction of the development of ES cells and their derivatives. These protocols mostly include the use of a combination of growth factors that control the differentiation of ES cells into highly specialized cells found in adult organs. The introduction of different growth factors into ES cells and their derivatives via viral gene transfer may greatly facilitate the optimization of these protocols. In this chapter, we describe a method based on herpes simplex virus type 1, which allows efficient gene transfer during several steps of a protocol, directed to obtain neural progenitors from ES cells. This protocol therefore may allow the study of potential factors influencing the cell fate or differentiation of ES cells and their derivatives.

This record has no associated files available for download.

More information

Published date: 15 February 2006
Keywords: human, stem-cells, germany, adult, pluripotent stem cells, mice, cell differentiation, proteins, herpesvirus 1, population, neurons, growth, transplantation, therapy, protein, genetic vectors

Identifiers

Local EPrints ID: 60167
URI: http://eprints.soton.ac.uk/id/eprint/60167
ISSN: 1064-3745
PURE UUID: 38849e71-2a3e-4a25-a2f9-d9c55af2d73d
ORCID for Dieter Riethmacher: ORCID iD orcid.org/0000-0002-4206-5529

Catalogue record

Date deposited: 05 Sep 2008
Last modified: 23 Jul 2022 01:58

Export record

Altmetrics

Contributors

Author: Dieter Riethmacher ORCID iD
Author: Filip Lim
Author: Thomas Schimmang

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×