The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Development of a transgenic green fluorescent protein lineage marker for steroidogenic factor 1

Development of a transgenic green fluorescent protein lineage marker for steroidogenic factor 1
Development of a transgenic green fluorescent protein lineage marker for steroidogenic factor 1
Knockout (KO) mice lacking steroidogenic factor 1 (SF-1, officially designated Nr5a1) have a complex phenotype that includes adrenal and gonadal agenesis, impaired function of pituitary gonadotropes, and abnormalities of the ventromedial hypothalamic nucleus (VMH). To develop a lineage marker for cells that express SF-1, we used bacterial artificial chromosome (BAC) transgenesis. A BAC fragment containing 50 kb of the mouse Nr5a1 gene was placed upstream of the coding sequence for enhanced green fluorescent protein (eGFP) and used to generate SF-1/eGFP transgenic mice. These sequences directed eGFP expression to multiple cell lineages that express SF-1, including steroidogenic cells of the adrenal cortex, testes, and ovaries, VMH neurons, and reticuloendothelial cells of the spleen. Despite the essential role of SF-1 in gonadotropes, eGFP was not expressed in the anterior pituitary. These studies show that 50 kb of the mouse Nr5a1 gene can target transgenic expression to multiple cell lineages that normally express SF-1. The SF-1/eGFP transgene provides a valuable tool to expand our understanding of the actions of SF-1 in endocrine development and function.
u.s.gov't, transgenic, cell lineage, testis, luminescent proteins, spleen, mice, ventromedial hypothalamic nucleus, chromosomes, research support, metabolism, p.h.s., bacterial, fushi tarazu transcription
0743-5800
497-504
Stallings, Nancy R.
9a5838d0-6c44-4858-b218-2bc0253202f4
Hanley, Neil A.
bf03f7bb-f377-44fb-8344-0bb1ca8b2ef9
Majdic, Gregor
d952bad6-60c5-43c0-86b9-03d95c141d7b
Zhao, Liping
a17ea846-4c1c-4047-b8dc-1145710f64ce
Bakke, Marit
68d36eb7-d69a-4ec6-bde9-bdf31c971551
Parker, Keith L.
a15d4ab4-1bf6-4294-bda3-ee68245d8303
Stallings, Nancy R.
9a5838d0-6c44-4858-b218-2bc0253202f4
Hanley, Neil A.
bf03f7bb-f377-44fb-8344-0bb1ca8b2ef9
Majdic, Gregor
d952bad6-60c5-43c0-86b9-03d95c141d7b
Zhao, Liping
a17ea846-4c1c-4047-b8dc-1145710f64ce
Bakke, Marit
68d36eb7-d69a-4ec6-bde9-bdf31c971551
Parker, Keith L.
a15d4ab4-1bf6-4294-bda3-ee68245d8303

Stallings, Nancy R., Hanley, Neil A., Majdic, Gregor, Zhao, Liping, Bakke, Marit and Parker, Keith L. (2002) Development of a transgenic green fluorescent protein lineage marker for steroidogenic factor 1. Endocrine Research, 28 (4), 497-504. (doi:10.1081/ERC-120016829).

Record type: Article

Abstract

Knockout (KO) mice lacking steroidogenic factor 1 (SF-1, officially designated Nr5a1) have a complex phenotype that includes adrenal and gonadal agenesis, impaired function of pituitary gonadotropes, and abnormalities of the ventromedial hypothalamic nucleus (VMH). To develop a lineage marker for cells that express SF-1, we used bacterial artificial chromosome (BAC) transgenesis. A BAC fragment containing 50 kb of the mouse Nr5a1 gene was placed upstream of the coding sequence for enhanced green fluorescent protein (eGFP) and used to generate SF-1/eGFP transgenic mice. These sequences directed eGFP expression to multiple cell lineages that express SF-1, including steroidogenic cells of the adrenal cortex, testes, and ovaries, VMH neurons, and reticuloendothelial cells of the spleen. Despite the essential role of SF-1 in gonadotropes, eGFP was not expressed in the anterior pituitary. These studies show that 50 kb of the mouse Nr5a1 gene can target transgenic expression to multiple cell lineages that normally express SF-1. The SF-1/eGFP transgene provides a valuable tool to expand our understanding of the actions of SF-1 in endocrine development and function.

This record has no associated files available for download.

More information

Published date: December 2002
Keywords: u.s.gov't, transgenic, cell lineage, testis, luminescent proteins, spleen, mice, ventromedial hypothalamic nucleus, chromosomes, research support, metabolism, p.h.s., bacterial, fushi tarazu transcription

Identifiers

Local EPrints ID: 60256
URI: http://eprints.soton.ac.uk/id/eprint/60256
ISSN: 0743-5800
PURE UUID: cf1e7c7b-7d9f-4c00-a815-4543bf7114b4

Catalogue record

Date deposited: 08 Sep 2008
Last modified: 08 Jan 2022 19:06

Export record

Altmetrics

Contributors

Author: Nancy R. Stallings
Author: Neil A. Hanley
Author: Gregor Majdic
Author: Liping Zhao
Author: Marit Bakke
Author: Keith L. Parker

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×