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The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins

The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins
The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins
It is unclear whether the rate of weight loss, independent of magnitude, affects whole body protein metabolism and the synthesis and plasma concentrations of specific hepatic secretory proteins. We examined 1) whether lean men losing weight rapidly (starvation) show greater changes in whole body protein kinetics, synthesis, and circulating concentrations of selected hepatic secretory proteins than those losing the same amount of weight more slowly [very low energy diet (VLED)]; and 2) whether plasma concentrations and synthetic rates of these proteins are related. Whole body protein kinetics were measured using [1-(13)C]leucine in 11 lean men (6 starvation, 5 VLED). Fractional and absolute synthetic rates of HDL-apolipoprotein A1 (apoA1), retinol binding protein, transthyretin, alpha(1)-antitrypsin (alpha(1)-AT), and transferrin were measured using a prime-constant intravenous infusion of [(13)C(2)]glycine. Compared with VLED group, the starvation group showed greater increases (at a 5% weight loss) in whole body protein oxidation (P < 0.05); fractional synthetic rates of HDL-apoA1 (25.3 vs. -1.52%; P = 0.003) and retinol binding protein (30.6 vs. 7.1%; P = 0.007); absolute synthetic rates of HDL-apoA1 (7.1 vs. -3.8 mg.kg(-1).day(-1); P = 0.003) and alpha(1)-AT (17.8 vs. 3.6 mg.kg(-1).day(-1); P = 0.02); and plasma concentration of alpha(1)-AT (P = 0.025). Relationships between synthetic rates and plasma concentrations varied between the secreted proteins. It is concluded that synthetic rates of hepatic secreted proteins in lean men are more closely related to the rate than the magnitude of weight loss. Changes in concentration of these secreted proteins can occur independently of changes in synthetic rates, and vice versa
protein turnover, plasma proteins, very low energy diet
0193-1849
E1580-E1589
Afolabi, Paul R.
757e7f01-664c-493e-bc51-c6a2c933dc22
Jahoor, Farook
7b3a0b55-f1a0-4dbd-bc48-d031a87a4ef0
Jackson, Alan A.
c9a12d7c-b4d6-4c92-820e-890a688379ef
Stubbs, James
98e05bf8-df52-4c40-86e3-7dcc7036f457
Johnstone, Alexander M.
25353253-5b72-441e-a0b3-172ac769fbe4
Faber, Peter
f0ae92d4-e429-4bf7-8efb-828249f32b54
Lobley, Gerald
fb77eba7-2378-4136-add8-7fff49114db0
Gibney, Eileen
b5ded52b-bf90-44c2-8d1e-ed5e4742bebb
Elia, Marinos
964bf436-e623-46d6-bc3f-5dd04c9ef4c1
Afolabi, Paul R.
757e7f01-664c-493e-bc51-c6a2c933dc22
Jahoor, Farook
7b3a0b55-f1a0-4dbd-bc48-d031a87a4ef0
Jackson, Alan A.
c9a12d7c-b4d6-4c92-820e-890a688379ef
Stubbs, James
98e05bf8-df52-4c40-86e3-7dcc7036f457
Johnstone, Alexander M.
25353253-5b72-441e-a0b3-172ac769fbe4
Faber, Peter
f0ae92d4-e429-4bf7-8efb-828249f32b54
Lobley, Gerald
fb77eba7-2378-4136-add8-7fff49114db0
Gibney, Eileen
b5ded52b-bf90-44c2-8d1e-ed5e4742bebb
Elia, Marinos
964bf436-e623-46d6-bc3f-5dd04c9ef4c1

Afolabi, Paul R., Jahoor, Farook, Jackson, Alan A., Stubbs, James, Johnstone, Alexander M., Faber, Peter, Lobley, Gerald, Gibney, Eileen and Elia, Marinos (2007) The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins. American Journal of Physiology: Endocrinology and Metabolism, 293 (6), E1580-E1589. (doi:10.1152/ajpendo.00169.2007).

Record type: Article

Abstract

It is unclear whether the rate of weight loss, independent of magnitude, affects whole body protein metabolism and the synthesis and plasma concentrations of specific hepatic secretory proteins. We examined 1) whether lean men losing weight rapidly (starvation) show greater changes in whole body protein kinetics, synthesis, and circulating concentrations of selected hepatic secretory proteins than those losing the same amount of weight more slowly [very low energy diet (VLED)]; and 2) whether plasma concentrations and synthetic rates of these proteins are related. Whole body protein kinetics were measured using [1-(13)C]leucine in 11 lean men (6 starvation, 5 VLED). Fractional and absolute synthetic rates of HDL-apolipoprotein A1 (apoA1), retinol binding protein, transthyretin, alpha(1)-antitrypsin (alpha(1)-AT), and transferrin were measured using a prime-constant intravenous infusion of [(13)C(2)]glycine. Compared with VLED group, the starvation group showed greater increases (at a 5% weight loss) in whole body protein oxidation (P < 0.05); fractional synthetic rates of HDL-apoA1 (25.3 vs. -1.52%; P = 0.003) and retinol binding protein (30.6 vs. 7.1%; P = 0.007); absolute synthetic rates of HDL-apoA1 (7.1 vs. -3.8 mg.kg(-1).day(-1); P = 0.003) and alpha(1)-AT (17.8 vs. 3.6 mg.kg(-1).day(-1); P = 0.02); and plasma concentration of alpha(1)-AT (P = 0.025). Relationships between synthetic rates and plasma concentrations varied between the secreted proteins. It is concluded that synthetic rates of hepatic secreted proteins in lean men are more closely related to the rate than the magnitude of weight loss. Changes in concentration of these secreted proteins can occur independently of changes in synthetic rates, and vice versa

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Published date: 2007
Keywords: protein turnover, plasma proteins, very low energy diet

Identifiers

Local EPrints ID: 60856
URI: https://eprints.soton.ac.uk/id/eprint/60856
ISSN: 0193-1849
PURE UUID: 3f7cca36-f470-4758-9b94-2609d27e5adb
ORCID for Paul R. Afolabi: ORCID iD orcid.org/0000-0002-0553-1578

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Date deposited: 10 Sep 2008
Last modified: 20 Jul 2019 01:17

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Contributors

Author: Paul R. Afolabi ORCID iD
Author: Farook Jahoor
Author: Alan A. Jackson
Author: James Stubbs
Author: Alexander M. Johnstone
Author: Peter Faber
Author: Gerald Lobley
Author: Eileen Gibney
Author: Marinos Elia

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