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Elevated BNP expression in mouse offspring left ventricles after protein restriction in utero

Elevated BNP expression in mouse offspring left ventricles after protein restriction in utero
Elevated BNP expression in mouse offspring left ventricles after protein restriction in utero
Objectives: We have previously shown that cyclin G1 expression is reduced in fetal hearts after in utero protein restriction (PR) suggesting reduced cardiac cell cycle. However no difference in cyclin G1 expression was seen in adult offspring hearts. We hypothesised that the hearts of adult PR group should be under greater stress to maintain cardiac output. We therefore measured brain natriuretic peptide (BNP) expression in fetal hearts and left ventricles of adult offspring in the control (C) and PR groups because BNP is a marker of left ventricular dysfunction during volume overload or cardiac fibrosis (Nishikimi et al. Cardiovasc Res. 2006).
Methods and results: Pregnant CD1 mice were placed on C (18% casein) or PR (9% casein) diet. Fetal hearts were collected on day 12 of gestation (C, n =11, PR, n =10) and the left ventricles (LV) of adult offspring at 6 months (C, n =17, PR, n =17). Fetal heart BNP mRNA expression relative to unit total RNA as measured by real-time PCR was similar in C and PR (C, 0.858F0.104 vs. PR, 0.761F0.096, p =NS). However, BNP expression in adult LV was greater in the PR than C (C, 7.043F0.68 vs. PR, 11.012F1.54, p =0.04).
Conclusion: These results indicate that protein restriction in pregnancy induces cellular changes (indicated by cyclin G1 changes) in the fetal heart which places it under stress in adulthood (elevated BNP production). Because BNP can suppress ventricular remodelling, we are presently investigating cardiac structural changes to assess whether these alterations are adaptive or maladaptive.
in-utero, protein, expression
0378-3782
516-517
Asopa, S.
a6e767da-b59c-461c-96f9-fab2ecf92b0b
Anthony, F.W.
398b8bed-237e-4fad-a77d-89266fb00bc0
Cagampang, F.R.A.
7cf57d52-4a65-4554-8306-ed65226bc50e
Terroni, P.L.
854be41d-2295-4155-a3a3-b6e2f3092a25
Ohri, S.K.
8aa5698c-78cf-4f59-a5af-5afa46f0348c
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Asopa, S.
a6e767da-b59c-461c-96f9-fab2ecf92b0b
Anthony, F.W.
398b8bed-237e-4fad-a77d-89266fb00bc0
Cagampang, F.R.A.
7cf57d52-4a65-4554-8306-ed65226bc50e
Terroni, P.L.
854be41d-2295-4155-a3a3-b6e2f3092a25
Ohri, S.K.
8aa5698c-78cf-4f59-a5af-5afa46f0348c
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f

Asopa, S., Anthony, F.W., Cagampang, F.R.A., Terroni, P.L., Ohri, S.K. and Hanson, M.A. (2006) Elevated BNP expression in mouse offspring left ventricles after protein restriction in utero. Early Human Development, 82 (8), 516-517.

Record type: Article

Abstract

Objectives: We have previously shown that cyclin G1 expression is reduced in fetal hearts after in utero protein restriction (PR) suggesting reduced cardiac cell cycle. However no difference in cyclin G1 expression was seen in adult offspring hearts. We hypothesised that the hearts of adult PR group should be under greater stress to maintain cardiac output. We therefore measured brain natriuretic peptide (BNP) expression in fetal hearts and left ventricles of adult offspring in the control (C) and PR groups because BNP is a marker of left ventricular dysfunction during volume overload or cardiac fibrosis (Nishikimi et al. Cardiovasc Res. 2006).
Methods and results: Pregnant CD1 mice were placed on C (18% casein) or PR (9% casein) diet. Fetal hearts were collected on day 12 of gestation (C, n =11, PR, n =10) and the left ventricles (LV) of adult offspring at 6 months (C, n =17, PR, n =17). Fetal heart BNP mRNA expression relative to unit total RNA as measured by real-time PCR was similar in C and PR (C, 0.858F0.104 vs. PR, 0.761F0.096, p =NS). However, BNP expression in adult LV was greater in the PR than C (C, 7.043F0.68 vs. PR, 11.012F1.54, p =0.04).
Conclusion: These results indicate that protein restriction in pregnancy induces cellular changes (indicated by cyclin G1 changes) in the fetal heart which places it under stress in adulthood (elevated BNP production). Because BNP can suppress ventricular remodelling, we are presently investigating cardiac structural changes to assess whether these alterations are adaptive or maladaptive.

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More information

Published date: August 2006
Additional Information: Abstracts of the 4th World Congress on Developmental Origins of Health and Disease (DOHaD), 13–16 September 2006, Educatorium, University of Utrecht, The Netherlands
Keywords: in-utero, protein, expression
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 60875
URI: https://eprints.soton.ac.uk/id/eprint/60875
ISSN: 0378-3782
PURE UUID: 0fe36b9e-28c0-4a2b-baf0-a996199430d3
ORCID for F.R.A. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853
ORCID for M.A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X

Catalogue record

Date deposited: 23 Sep 2008
Last modified: 16 Mar 2019 01:34

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Contributors

Author: S. Asopa
Author: F.W. Anthony
Author: P.L. Terroni
Author: S.K. Ohri
Author: M.A. Hanson ORCID iD

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