Asopa, S., Anthony, F.W., Cagampang, F.R.A., Terroni, P.L., Bhattacharya, S., Ohri, S.K. and Hanson, M.A.
Impact on the developmental profile of the murine heart by maternal protein restriction during preganancy
Early Human Development, 83, (Supplement1), . (doi:10.1016/S0378-3782(07)70186-X).
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Aims: We hypothesised that intrauterine environmental factors
such as undernutrition may have an impact on cardiac development
in prenatal life, leading to cardiac hypertrophy. In this study, we
examined the effects of maternal dietary protein restriction (PR)
during pregnancy on fetal heart development in the mouse.
Study design and Subjects: CD1 mice were placed on control
C (18% casein) or protein restricted PR (9% casein) diet during
pregnancy. Fetal hearts were collected on day 12 of gestation and
the left ventricles (LV) of adult offspring at 6 months.
Outcome Measures: p53, e2f1 mRNA expression was measured by
real time RT PCR, fetal heart ventricular volumes and surface area
of by MRI.
Results: No differences were demonstrated in p53 and E2f1
expression in fetal heart or adult LV. MRI revealed smaller hearts in
the PR group, both for surface area (PR, 13831±68 vs. C, 16356±37
mm2, p<0.01) and ventricular volume (PR, 22114±43 v C, 27404±10
Conclusion: These results indicate that protein restriction during
pregnancy leads to a smaller fetal heart (MRI scan), perhaps due
to its effect on cell allocation and division. Lack of difference in
the expression of p53 and E2f1, genes involved in cell inhibition
and apoptosis and cell proliferation, respectively in either group
suggest that changes in fetal heart size are due to the reduction
of cardiomyocyte growth (supported by lower cyclin g1 expression
previously described) in the PR group.
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