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The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs

The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs
The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs
Until now, nanotopography has been considered in 2D construct designs. This has been due to fabrication limitations with traditional lithographic processes relying on the ability to focus radiation that will expose a radiation sensitive resist (e.g. photolithography and electron beam lithography). More recently, alternative methods that offer rapid and cheap nanofabrication have been developed; such methods include polymer demixing and colloidal lithography. Polymer demixing in 2D has relied on spin casting of polymer blends-such as polystyrene and polybromostyrene in a solvent such as toluene. As the solvent evaporates, the polymers phase separate and form nanoislands. In this study, the polymer blend solution has been blown through fine tubes and allowed to demix, thus providing 3D constructs for cell biology. The ability to fabricate in tubes may be useful in many applications, for example stents, conduits, and bone repair (when considering structures such as Haversian tubes and Volkmann's canals). As proof of concept, human osteoprogenitor cells have been used to test the cell response to the nanopatterned tubes. The results show that nanofeatures of size X, diameter Y, and spacing Z decrease cell spreading, reduce cytoskeletal organization, and increase endocytotic activity within the cells
chemistry, osteoblasts, bone marrow, activity, cell culture techniques, stents, methods, cells, stem cells, size, human, humans, tissue engineering, bone regeneration, polymers, female, toluene, cell adhesion, metabolism, polystyrenes, bone, cytology, aged, clathrin, bone marrow cells
1549-3296
431-439
Berry, C.C.
9d729b5b-d244-42bc-a44e-ad74874d9d2d
Dalby, M.J.
b1f2e705-d463-433a-9e27-bcfe17568ddb
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
McCloy, D.
53d56283-509e-40c2-9218-0c30083af53d
Affrosman, S.
e56baf4c-c33b-44e8-8be5-461859d3275f
Berry, C.C.
9d729b5b-d244-42bc-a44e-ad74874d9d2d
Dalby, M.J.
b1f2e705-d463-433a-9e27-bcfe17568ddb
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
McCloy, D.
53d56283-509e-40c2-9218-0c30083af53d
Affrosman, S.
e56baf4c-c33b-44e8-8be5-461859d3275f

Berry, C.C., Dalby, M.J., Oreffo, R.O., McCloy, D. and Affrosman, S. (2006) The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs. Journal of Biomedical Materials Research Part A, 79 (2), 431-439.

Record type: Article

Abstract

Until now, nanotopography has been considered in 2D construct designs. This has been due to fabrication limitations with traditional lithographic processes relying on the ability to focus radiation that will expose a radiation sensitive resist (e.g. photolithography and electron beam lithography). More recently, alternative methods that offer rapid and cheap nanofabrication have been developed; such methods include polymer demixing and colloidal lithography. Polymer demixing in 2D has relied on spin casting of polymer blends-such as polystyrene and polybromostyrene in a solvent such as toluene. As the solvent evaporates, the polymers phase separate and form nanoislands. In this study, the polymer blend solution has been blown through fine tubes and allowed to demix, thus providing 3D constructs for cell biology. The ability to fabricate in tubes may be useful in many applications, for example stents, conduits, and bone repair (when considering structures such as Haversian tubes and Volkmann's canals). As proof of concept, human osteoprogenitor cells have been used to test the cell response to the nanopatterned tubes. The results show that nanofeatures of size X, diameter Y, and spacing Z decrease cell spreading, reduce cytoskeletal organization, and increase endocytotic activity within the cells

Full text not available from this repository.

More information

Published date: 2006
Keywords: chemistry, osteoblasts, bone marrow, activity, cell culture techniques, stents, methods, cells, stem cells, size, human, humans, tissue engineering, bone regeneration, polymers, female, toluene, cell adhesion, metabolism, polystyrenes, bone, cytology, aged, clathrin, bone marrow cells

Identifiers

Local EPrints ID: 60913
URI: http://eprints.soton.ac.uk/id/eprint/60913
ISSN: 1549-3296
PURE UUID: a7fdabc2-8552-4314-9aa3-1417924704d0
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 12 Sep 2008
Last modified: 17 Dec 2019 01:53

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