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Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates

Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates
Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates
The separate influence of topographical and chemical cues on cell attachment and spreading are well documented; however, that of duel-cue substrates is less so. In this study graft copolymers that sterically stabilize biological surfaces were employed alongside nanotopographical features fabricated by colloidal lithography. This resulted in the production of a range of substrates whereby the effect of chemistry and or topography on both on human fibroblast and bone marrow cell adhesion and spreading could be observed. The current studies indicate an enhancement of cell response as a consequence of modifications in material topography, whereas the current selected chemical cues inhibited cell function. Critically, in combination, topography modulated the effects of chemical environment.
cytology, cell survival, cultured, research, humans, human, cues, fibroblasts, methods, proteins, surface properties, tissue engineering, environment, cell adhesion, substrate specificity, cell proliferation, cells, bone, ultrastructure, nanostructures, biocompatible materials, bone marrow, cell culture techniques, metabolism, function, materials testing, bone marrow cells, protein, physiology, chemistry
201-209
Berry, C.C.
9d729b5b-d244-42bc-a44e-ad74874d9d2d
Curtis, A.S.G.
1d1365b5-4265-4c9d-b8b2-594f92427f99
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Agheli, H.D.S.
04894fdf-b638-4cd1-99da-5581d1dd3e3d
Sutherland, D.S.
7cc58a21-9969-460b-858a-f20f7ff521d7
Berry, C.C.
9d729b5b-d244-42bc-a44e-ad74874d9d2d
Curtis, A.S.G.
1d1365b5-4265-4c9d-b8b2-594f92427f99
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Agheli, H.D.S.
04894fdf-b638-4cd1-99da-5581d1dd3e3d
Sutherland, D.S.
7cc58a21-9969-460b-858a-f20f7ff521d7

Berry, C.C., Curtis, A.S.G., Oreffo, R.O.C., Agheli, H.D.S. and Sutherland, D.S. (2007) Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates. IEEE Transactions on Nanobioscience, 6 (3), 201-209. (doi:10.1109/TNB.2007.903457).

Record type: Article

Abstract

The separate influence of topographical and chemical cues on cell attachment and spreading are well documented; however, that of duel-cue substrates is less so. In this study graft copolymers that sterically stabilize biological surfaces were employed alongside nanotopographical features fabricated by colloidal lithography. This resulted in the production of a range of substrates whereby the effect of chemistry and or topography on both on human fibroblast and bone marrow cell adhesion and spreading could be observed. The current studies indicate an enhancement of cell response as a consequence of modifications in material topography, whereas the current selected chemical cues inhibited cell function. Critically, in combination, topography modulated the effects of chemical environment.

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More information

Published date: September 2007
Keywords: cytology, cell survival, cultured, research, humans, human, cues, fibroblasts, methods, proteins, surface properties, tissue engineering, environment, cell adhesion, substrate specificity, cell proliferation, cells, bone, ultrastructure, nanostructures, biocompatible materials, bone marrow, cell culture techniques, metabolism, function, materials testing, bone marrow cells, protein, physiology, chemistry

Identifiers

Local EPrints ID: 60914
URI: http://eprints.soton.ac.uk/id/eprint/60914
PURE UUID: c6117f08-34c3-4217-ae9a-ee5ea23f4d39
ORCID for R.O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 23 Sep 2008
Last modified: 16 Mar 2024 03:11

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Contributors

Author: C.C. Berry
Author: A.S.G. Curtis
Author: R.O.C. Oreffo ORCID iD
Author: H.D.S. Agheli
Author: D.S. Sutherland

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