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Placental materno-fetal transfer of serine by amino acid exchangers

Cleal, J.K., Brownbill, P., Godfrey, K.M., Cameron, I.T., Sibley, C.P., Hanson, M.A. and Lewis, R.M. (2006) Placental materno-fetal transfer of serine by amino acid exchangers Placenta, 27, (9-10), p.34.

Record type: Article


Objectives: The mechanisms mediating amino acid transport across the basal membrane of the placental syncytiotrophoblast into the fetal circulation are not well understood. Our previous data indicate that amino acid exchangers mediate serine transport into the fetoplacental circulation in exchange for alanine but not for glutamate. This study characterises amino acid stimulation of serine transfer into the fetoplacental circulation by exchange.
Methods: Human placentas (n = 4) were collected within 30 minutes of delivery and an intact cotyledon was perfused with a modified Earl’s bicarbonate buffer. The maternal arterial circulation was perfused with 50 ?mol /1 L-serine & glycine, 0.6 ?mol /1 14Cserine and 20 ?mol /1 3H-glycine. Amino acid [12.5 ?mol] boluses were administered to the fetal side inflow perfusate. 14C-serine and 3H-glycine were measured in maternal and fetal venous samples by dual label liquid scintillation counting. Data (mean ± SEM) were expressed as area under the curve (AUC) and analysed by one way ANOVA.
Results: Fetal side boluses of serine leucine threonine tryptophan and glutamine all resulted in increased serine release (AUC values 242 ± 34, 132 ± 33, 309 ± 69, 85 ± 21 and 216 ± 82 nmol respectively). Following a fetal side bolus of glutamate, BCH or lysine there was no significant increase in transfer of serine. Glycine release was not significantly altered following any of the boluses.
Conclusion: This study demonstrates that serine is transported into the fetoplacental circulation by amino acid exchangers in response to a range of substrates, but not lysine or BCH, substrates of systems y+L and L. This is consistent with serine transport by Systems ASC and b0+ but not Systems y+L and L.

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Published date: October 2006
Additional Information: Abstracts for the forthcoming International Federation of Placenta Associations Meeting (Pages A1-A57)
Keywords: acid, serine


Local EPrints ID: 60995
ISSN: 0143-4004
PURE UUID: 61265adf-ff6a-49a5-9d36-8d16bdb051e2
ORCID for K.M. Godfrey: ORCID iD
ORCID for I.T. Cameron: ORCID iD
ORCID for R.M. Lewis: ORCID iD

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Date deposited: 29 Sep 2008
Last modified: 28 Aug 2017 20:43

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Author: J.K. Cleal
Author: P. Brownbill
Author: K.M. Godfrey ORCID iD
Author: I.T. Cameron ORCID iD
Author: C.P. Sibley
Author: M.A. Hanson
Author: R.M. Lewis ORCID iD

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