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Osteoprogenitor response to semi-ordered and random nanotopographies

Osteoprogenitor response to semi-ordered and random nanotopographies
Osteoprogenitor response to semi-ordered and random nanotopographies
In bone tissue engineering, it is desirable to use materials to control the differentiation of mesenchymal stem cell populations in order to gain direct bone apposition to implant materials. It has been known for a number of years that microtopography can alter cell adhesion, proliferation and gene expression. More recently, the literature reveals that nanotopography is also of importance. Here, the reaction of primary human osteoprogenitor cell populations to nanotopographies down to 10 nm in size is considered. The topographies were originally produced by colloidal lithography and polymer demixing on silicon and then embossed (through an intermediate nickel shim) into polymethylmethacrylate. The biological testing considered cell morphology (image analysis of cell spreading and scanning electron microscopy), cell cytoskleton and adhesion formation (fluorescent staining of actin, tubulin, vimentin and vinculin) and then subsequent cell growth and differentiation (fluorescent staining of osteocalcin and osteopontin). The results demonstrated that the nanotopographies stimulated the osteoprogenitor cell differentiation towards an osteoblastic phenotype.
size, bone, surface properties, gene, research, human, cultured, polymethyl methacrylate, methods, cell differentiation, osteoblasts, infection, bone substitutes, tissue engineering, osteogenesis, gene-expression, female, gene expression, growth, cell culture techniques, aged, cells, cell adhesion, materials testing, analysis, chemistry, hematopoietic stem cells, phenotype, physiology, humans, cell proliferation, osteocalcin, cytology, expression
0142-9612
2980-2987
Dalby, M.J.
b1f2e705-d463-433a-9e27-bcfe17568ddb
McCloy, D.
53d56283-509e-40c2-9218-0c30083af53d
Robertson, M.
3643ecd6-d3f9-4ccb-8eaf-040b028f5dad
Agheli, H.D.
5db027d9-fa2b-4cbf-b3c3-196a75ee0094
Affrossman, S.
603b0236-5a66-45ea-b134-489b3a4b6967
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, M.J.
b1f2e705-d463-433a-9e27-bcfe17568ddb
McCloy, D.
53d56283-509e-40c2-9218-0c30083af53d
Robertson, M.
3643ecd6-d3f9-4ccb-8eaf-040b028f5dad
Agheli, H.D.
5db027d9-fa2b-4cbf-b3c3-196a75ee0094
Affrossman, S.
603b0236-5a66-45ea-b134-489b3a4b6967
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Dalby, M.J., McCloy, D., Robertson, M., Agheli, H.D., Affrossman, S. and Oreffo, R.O. (2006) Osteoprogenitor response to semi-ordered and random nanotopographies. Biomaterials, 27 (15), 2980-2987. (doi:10.1016/j.biomaterials.2006.01.010).

Record type: Article

Abstract

In bone tissue engineering, it is desirable to use materials to control the differentiation of mesenchymal stem cell populations in order to gain direct bone apposition to implant materials. It has been known for a number of years that microtopography can alter cell adhesion, proliferation and gene expression. More recently, the literature reveals that nanotopography is also of importance. Here, the reaction of primary human osteoprogenitor cell populations to nanotopographies down to 10 nm in size is considered. The topographies were originally produced by colloidal lithography and polymer demixing on silicon and then embossed (through an intermediate nickel shim) into polymethylmethacrylate. The biological testing considered cell morphology (image analysis of cell spreading and scanning electron microscopy), cell cytoskleton and adhesion formation (fluorescent staining of actin, tubulin, vimentin and vinculin) and then subsequent cell growth and differentiation (fluorescent staining of osteocalcin and osteopontin). The results demonstrated that the nanotopographies stimulated the osteoprogenitor cell differentiation towards an osteoblastic phenotype.

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More information

Published date: 2006
Keywords: size, bone, surface properties, gene, research, human, cultured, polymethyl methacrylate, methods, cell differentiation, osteoblasts, infection, bone substitutes, tissue engineering, osteogenesis, gene-expression, female, gene expression, growth, cell culture techniques, aged, cells, cell adhesion, materials testing, analysis, chemistry, hematopoietic stem cells, phenotype, physiology, humans, cell proliferation, osteocalcin, cytology, expression

Identifiers

Local EPrints ID: 61036
URI: http://eprints.soton.ac.uk/id/eprint/61036
ISSN: 0142-9612
PURE UUID: b18d9309-c57d-467b-b5e3-868e05398974
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 02 Sep 2008
Last modified: 17 Dec 2019 01:53

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Contributors

Author: M.J. Dalby
Author: D. McCloy
Author: M. Robertson
Author: H.D. Agheli
Author: S. Affrossman
Author: R.O. Oreffo ORCID iD

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