Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells
Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells
Type I Collagen matrices of defined porosity, incorporating carbonate substituted hydroxyapatite (HA) crystals, were assessed for their ability to support osteo- and chondrogenic differentiation of human bone marrow stromal cells (HBMSCs). Collagen-HA composite scaffolds supported the osteogenic differentiation of HBMSCs both in vitro and in vivo as demonstrated by histological and micro-CT analyses indicating the extensive penetration of alkaline phosphatase expressing cells and new matrix synthesis with localised areas immunologically positive for osteocalcin. In vivo, extensive new osteoid formation of implant origin was observed in the areas of vasculature. Chondrogenic matrix synthesis was evidenced in the peripheral regions of pure collagen systems by an abundance of Sox9 expressing chondrocytes embedded within a proteoglycan and collagen II rich ECM. The introduction of microchannels to the scaffold architecture was seen to enhance chondrogenesis. Tissue specific gene expression and corresponding matrix synthesis indicate that collagen matrices support the growth and differentiation of HBMSCs and suggest the potential of this platform for understanding the ECM cues necessary for osteogenesis and chondrogenesis.
bone tissue engineering, cartilage tissue engineering, collagen, scaffold, biomimetic material
3105-3116
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Wahl, Denys A.
b6a642b7-087e-457b-9dba-a083fb99cc51
Lanham, Stuart A.
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Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Czernuszka, Jan T.
84648dff-9f64-4d03-b163-67180b1c2468
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
July 2008
Dawson, Jonathan I.
b220fe76-498d-47be-9995-92da6c289cf3
Wahl, Denys A.
b6a642b7-087e-457b-9dba-a083fb99cc51
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Czernuszka, Jan T.
84648dff-9f64-4d03-b163-67180b1c2468
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dawson, Jonathan I., Wahl, Denys A., Lanham, Stuart A., Kanczler, Janos M., Czernuszka, Jan T. and Oreffo, Richard O.C.
(2008)
Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells.
Biomaterials, 29 (21), .
(doi:10.1016/j.biomaterials.2008.03.040).
Abstract
Type I Collagen matrices of defined porosity, incorporating carbonate substituted hydroxyapatite (HA) crystals, were assessed for their ability to support osteo- and chondrogenic differentiation of human bone marrow stromal cells (HBMSCs). Collagen-HA composite scaffolds supported the osteogenic differentiation of HBMSCs both in vitro and in vivo as demonstrated by histological and micro-CT analyses indicating the extensive penetration of alkaline phosphatase expressing cells and new matrix synthesis with localised areas immunologically positive for osteocalcin. In vivo, extensive new osteoid formation of implant origin was observed in the areas of vasculature. Chondrogenic matrix synthesis was evidenced in the peripheral regions of pure collagen systems by an abundance of Sox9 expressing chondrocytes embedded within a proteoglycan and collagen II rich ECM. The introduction of microchannels to the scaffold architecture was seen to enhance chondrogenesis. Tissue specific gene expression and corresponding matrix synthesis indicate that collagen matrices support the growth and differentiation of HBMSCs and suggest the potential of this platform for understanding the ECM cues necessary for osteogenesis and chondrogenesis.
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Published date: July 2008
Keywords:
bone tissue engineering, cartilage tissue engineering, collagen, scaffold, biomimetic material
Identifiers
Local EPrints ID: 61042
URI: http://eprints.soton.ac.uk/id/eprint/61042
ISSN: 0142-9612
PURE UUID: 6c11b697-f589-4ec5-a304-7b9ed9bbd47e
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Date deposited: 02 Sep 2008
Last modified: 16 Mar 2024 03:57
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Contributors
Author:
Denys A. Wahl
Author:
Stuart A. Lanham
Author:
Janos M. Kanczler
Author:
Jan T. Czernuszka
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