Membrane fatty acids, oxidative burst and phagocytosis after enrichment of P388D1 monocyte/macrophages with essential 18-carbon fatty acids
Membrane fatty acids, oxidative burst and phagocytosis after enrichment of P388D1 monocyte/macrophages with essential 18-carbon fatty acids
The fatty acid composition of cell membranes can be modified in cell culture. The role of different fatty acid families in modulating phagocytosis and oxidative burst is not clear and therefore the influence of 18-carbon polyunsaturated fatty acids (PUFA) on these processes was examined. The mouse monocyte/macrophage line P388D1 was cultured in medium supplemented with 2 or 20 micromol/l 18:2n-6 (linoleic acid; LA) or 18:3n-3 (alpha-linolenic acid; LNA) and fatty acid enrichment of the cells was tested after 8 days. The macrophages were activated with phorbol ester in order to promote oxidative burst and intracellular dihydrorhodamine oxidation was determined. To test phagocytosis capacity uptake of fluorescence-labeled Escherichia coli was determined. Activation of the transcription factor nuclear factor (NF)-kappaB was also determined. Cells grown in medium with 20 micromol/l LA contained 2- to 3-fold more n-6 PUFA including 4-fold more arachidonic acid. Cells grown in medium with 20 micromol/l LNA contained 4-fold more n-3 PUFA. Both LA and LNA enhanced phagocytosis and decreased oxidative burst, with little difference between the fatty acids. NF-kappaB activation at 1 h post-stimulation was not affected by adding LA or LNA to the culture medium. We conclude that the fatty acid composition of macrophages influences their ability to phagocytose and mount oxidative burst.
animals, mice, acid, pharmacology, linoleic acid, cell membrane, humans, respiratory burst, nf-kappa b, chemistry, cell line, macrophages, unsaturated, oxidation-reduction, monocytes, drug effects, drug, physiology, phagocytosis, fatty acids, dose-response relationship, arachidonic acid, culture media, metabolism
155-162
Fuhrmann, Herbert
a77a035e-138e-4dd4-b5c7-969efa03d3db
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
West, Anette L.
c1923242-802f-4331-b743-31de45d3883c
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
June 2007
Fuhrmann, Herbert
a77a035e-138e-4dd4-b5c7-969efa03d3db
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
West, Anette L.
c1923242-802f-4331-b743-31de45d3883c
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fuhrmann, Herbert, Miles, Elizabeth A., West, Anette L. and Calder, Philip C.
(2007)
Membrane fatty acids, oxidative burst and phagocytosis after enrichment of P388D1 monocyte/macrophages with essential 18-carbon fatty acids.
Annals of Nutrition and Metabolism, 51 (2), .
(doi:10.1159/000103276).
Abstract
The fatty acid composition of cell membranes can be modified in cell culture. The role of different fatty acid families in modulating phagocytosis and oxidative burst is not clear and therefore the influence of 18-carbon polyunsaturated fatty acids (PUFA) on these processes was examined. The mouse monocyte/macrophage line P388D1 was cultured in medium supplemented with 2 or 20 micromol/l 18:2n-6 (linoleic acid; LA) or 18:3n-3 (alpha-linolenic acid; LNA) and fatty acid enrichment of the cells was tested after 8 days. The macrophages were activated with phorbol ester in order to promote oxidative burst and intracellular dihydrorhodamine oxidation was determined. To test phagocytosis capacity uptake of fluorescence-labeled Escherichia coli was determined. Activation of the transcription factor nuclear factor (NF)-kappaB was also determined. Cells grown in medium with 20 micromol/l LA contained 2- to 3-fold more n-6 PUFA including 4-fold more arachidonic acid. Cells grown in medium with 20 micromol/l LNA contained 4-fold more n-3 PUFA. Both LA and LNA enhanced phagocytosis and decreased oxidative burst, with little difference between the fatty acids. NF-kappaB activation at 1 h post-stimulation was not affected by adding LA or LNA to the culture medium. We conclude that the fatty acid composition of macrophages influences their ability to phagocytose and mount oxidative burst.
This record has no associated files available for download.
More information
Published date: June 2007
Keywords:
animals, mice, acid, pharmacology, linoleic acid, cell membrane, humans, respiratory burst, nf-kappa b, chemistry, cell line, macrophages, unsaturated, oxidation-reduction, monocytes, drug effects, drug, physiology, phagocytosis, fatty acids, dose-response relationship, arachidonic acid, culture media, metabolism
Identifiers
Local EPrints ID: 61120
URI: http://eprints.soton.ac.uk/id/eprint/61120
ISSN: 0250-6807
PURE UUID: fe88fe7f-826f-434f-a7e7-5ccac3dfc577
Catalogue record
Date deposited: 03 Sep 2008
Last modified: 16 Mar 2024 03:43
Export record
Altmetrics
Contributors
Author:
Herbert Fuhrmann
Author:
Anette L. West
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics