Gene therapy used for tissue engineering applications

Heyde, Mieke, Partridge, Kris A., Oreffo, Richard O.C., Howdle, Steven M., Shakesheff, Kevin M. and Garnett, Martin C. (2007) Gene therapy used for tissue engineering applications. Journal of Pharmacy and Pharmacology, 59 (3), 329-350.

Abstract

This review highlights the advances at the interface between tissue engineering and gene therapy. There are a large number of reports on gene therapy in tissue engineering, and these cover a huge range of different engineered tissues, different vectors, scaffolds and methodology. The review considers separately in-vitro and in-vivo gene transfer methods. The in-vivo gene transfer method is described first, using either viral or non-viral vectors to repair various tissues with and without the use of scaffolds. The use of a scaffold can overcome some of the challenges associated with delivery by direct injection. The ex-vivo method is described in the second half of the review. Attempts have been made to use this therapy for bone, cartilage, wound, urothelial, nerve tissue regeneration and for treating diabetes using viral or non-viral vectors. Again porous polymers can be used as scaffolds for cell transplantation. There are as yet few comparisons between these many different variables to show which is the best for any particular application. With few exceptions, all of the results were positive in showing some gene expression and some consequent effect on tissue growth and remodelling. Some of the principal advantages and disadvantages of various methods are discussed.

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Contributors

Author: Richard O.C. Oreffo

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