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The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation

The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation
The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation
The capacity to deliver, temporally, bioactive growth factors in combination with appropriate progenitor and stem cells to sites of tissue regeneration promoting angiogenesis and osteogenesis offers therapeutic opportunities in regenerative medicine. We have examined the bone regenerative potential of encapsulated vascular endothelial growth factor (VEGF(165)) biodegradable poly(DL-lactic acid) (PLA) scaffolds created using supercritical CO(2) fluid technology to encapsulate and release solvent-sensitive and thermolabile growth factors in combination with human bone marrow stromal cells (HBMSC) implanted in a mouse femur segmental defect (5 mm) for 4 weeks. HBMSC seeded on VEGF encapsulated PLA scaffolds showed significant bone regeneration in the femur segmental defect compared to the scaffold alone and scaffold seeded with HBMSC as analysed by indices of increased bone volume (BV mm(3)), trabecular number (Tb.N/mm) and reduced trabecular separation (Tb.Sp.mm) in the defect region using micro-computed tomography. Histological examination confirmed significant new bone matrix in the HBMSC seeded VEGF encapsulated scaffold group as evidenced by Sirius red/alcian blue and Goldner's trichrome staining and type I collagen immunocytochemistry expression in comparison to the other groups. These studies demonstrate the ability to deliver, temporally, a combination of VEGF released from scaffolds with seeded HBMSC to sites of bone defects, results in enhanced regeneration of a bone defect
male, cultured, origins, administration & dosage, absorbable implants, stromal cells, combined modality therapy, vascular endothelial growth factor a, health, animals, protein, human, cells, drug carriers, human development, growth, stem cells, vascular, humans, lactic acid, disease, development, acid, research, expression, nude, bone, polymers, mesenchymal stem cell transplantation, bone marrow, mice, developmental origins, femoral fractures, femur, methods, pathology, osteogenesis, chemistry, therapy, treatment outcome, drug effects, regeneration, bone regeneration
0142-9612
1892-1900
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Ginty, P.J.
af21d284-462a-4148-af85-eed1646f3b8e
Barry, J.J.
20084b5a-e861-42cf-b77b-a2ef507ed1f7
Clarke, N.M.
76688c21-d51e-48fa-a84d-deec66baf8ac
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Shakesheff, K.M.
9e3f7c5c-9191-40b6-b7b3-f454110a7950
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Ginty, P.J.
af21d284-462a-4148-af85-eed1646f3b8e
Barry, J.J.
20084b5a-e861-42cf-b77b-a2ef507ed1f7
Clarke, N.M.
76688c21-d51e-48fa-a84d-deec66baf8ac
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Shakesheff, K.M.
9e3f7c5c-9191-40b6-b7b3-f454110a7950
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Kanczler, J.M., Ginty, P.J., Barry, J.J., Clarke, N.M., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O. (2008) The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation. Biomaterials, 29 (12), 1892-1900. (doi:10.1016/j.biomaterials.2007.12.031).

Record type: Article

Abstract

The capacity to deliver, temporally, bioactive growth factors in combination with appropriate progenitor and stem cells to sites of tissue regeneration promoting angiogenesis and osteogenesis offers therapeutic opportunities in regenerative medicine. We have examined the bone regenerative potential of encapsulated vascular endothelial growth factor (VEGF(165)) biodegradable poly(DL-lactic acid) (PLA) scaffolds created using supercritical CO(2) fluid technology to encapsulate and release solvent-sensitive and thermolabile growth factors in combination with human bone marrow stromal cells (HBMSC) implanted in a mouse femur segmental defect (5 mm) for 4 weeks. HBMSC seeded on VEGF encapsulated PLA scaffolds showed significant bone regeneration in the femur segmental defect compared to the scaffold alone and scaffold seeded with HBMSC as analysed by indices of increased bone volume (BV mm(3)), trabecular number (Tb.N/mm) and reduced trabecular separation (Tb.Sp.mm) in the defect region using micro-computed tomography. Histological examination confirmed significant new bone matrix in the HBMSC seeded VEGF encapsulated scaffold group as evidenced by Sirius red/alcian blue and Goldner's trichrome staining and type I collagen immunocytochemistry expression in comparison to the other groups. These studies demonstrate the ability to deliver, temporally, a combination of VEGF released from scaffolds with seeded HBMSC to sites of bone defects, results in enhanced regeneration of a bone defect

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More information

e-pub ahead of print date: 29 January 2008
Published date: April 2008
Keywords: male, cultured, origins, administration & dosage, absorbable implants, stromal cells, combined modality therapy, vascular endothelial growth factor a, health, animals, protein, human, cells, drug carriers, human development, growth, stem cells, vascular, humans, lactic acid, disease, development, acid, research, expression, nude, bone, polymers, mesenchymal stem cell transplantation, bone marrow, mice, developmental origins, femoral fractures, femur, methods, pathology, osteogenesis, chemistry, therapy, treatment outcome, drug effects, regeneration, bone regeneration
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 61273
URI: http://eprints.soton.ac.uk/id/eprint/61273
ISSN: 0142-9612
PURE UUID: ee5a967a-285d-42ee-a638-3e6f3f38ab40
ORCID for J.M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 02 Sep 2008
Last modified: 11 Jul 2024 01:43

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Contributors

Author: J.M. Kanczler ORCID iD
Author: P.J. Ginty
Author: J.J. Barry
Author: N.M. Clarke
Author: S.M. Howdle
Author: K.M. Shakesheff
Author: R.O. Oreffo ORCID iD

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