The University of Southampton
University of Southampton Institutional Repository

Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response

Record type: Article

Monocytes/macrophages are key orchestrators of inflammation and are involved in the pathogenesis of chronic inflammatory disorders, including atherosclerosis. (n-3) Fatty acids, found in fish oil, have been shown to have protective effects in such disorders. To investigate possible modes of action, we used a monocyte:endothelial cell (EC) coculture model to investigate the pro-inflammatory potential of monocytes. Monocytes were isolated from the blood of donors with peripheral arterial disease (PAD) or control donors, before and after a 12-wk supplementation of their diet with fish oil. The monocytes were cultured with human umbilical vein EC (HUVEC) for 24 h, after which the ability of the HUVEC to recruit flowing neutrophils was tested. Monocytes from either group of donors stimulated the EC to support the adhesion and migration of neutrophils. Fish oil supplementation reduced the potency of monocytes from normal subjects, but not those from patients with PAD, to induce recruitment. Concurrent medication may have acted as a complicating factor. On subgroup analysis, only those free of medication showed a significant effect of fish oil. Responses before or after supplementation were not closely linked to patterns of secretion of cytokines by cultured monocytes, tested in parallel monocultures. These results suggest that fish oil can modulate the ability of monocytes to stimulate EC and that this might contribute to their protective effects against chronic inflammatory disorders. Benefits, however, may depend on existing medical status and on other treatments being received.

Full text not available from this repository.

Citation

Luu, Nguyet-Thin, Madden, Jackie, Calder, Philip C., Grimble, Robert F., Shearman, Cliff P., Chan, Tim, Dastur, Neville, Howell, William M., Rainger, G. Ed and Nash, Gerard B. (2007) Dietary supplementation with fish oil modifies the ability of human monocytes to induce an inflammatory response Journal of Nutrition, 137, (12), pp. 2769-2774.

More information

Published date: December 2007
Organisations: Dev Origins of Health & Disease

Identifiers

Local EPrints ID: 61348
URI: http://eprints.soton.ac.uk/id/eprint/61348
ISSN: 0022-3166
PURE UUID: 75fffc9f-0048-4a11-9377-1d253db64a4a

Catalogue record

Date deposited: 23 Sep 2008
Last modified: 17 Jul 2017 14:22

Export record

Contributors

Author: Nguyet-Thin Luu
Author: Jackie Madden
Author: Robert F. Grimble
Author: Tim Chan
Author: Neville Dastur
Author: William M. Howell
Author: G. Ed Rainger
Author: Gerard B. Nash

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×