Decreased tumor growth in Walker 256 tumor-bearing rats chronically supplemented with fish oil involves COX-2 and PGE2 reduction associated with apoptosis and increased peroxidation
Decreased tumor growth in Walker 256 tumor-bearing rats chronically supplemented with fish oil involves COX-2 and PGE2 reduction associated with apoptosis and increased peroxidation
Many studies have shown that addition of fish oil (FO) to the diet reduces tumor growth but the mechanism(s) of action involved is (are) still unknown. In this study, we examine some possible mechanisms in tumor-bearing rats chronically supplemented with FO. Male Wistar rats (21 days old) were fed with regular chow and supplemented with coconut or FO (1g/kg body weight) until they reached 70 days of age. Then, they were inoculated with a suspension of Walker 256 ascitic tumor cells (2 x 10(7)ml) and after 14 days they were killed. Supplementation with FO resulted in significantly lower tumor weight, greater tumor cell apoptosis, lower ex vivo tumor cell proliferation, a higher tumor content of lipid peroxides, lower expression of cyclooxygenase-2 (COX-2) in tumor tissue and a lower plasma concentration of prostaglandin E2 than observed in rats fed regular chow or supplemented with coconut oil. These results suggest that reduction of tumor growth by FO involves an increase in apoptosis and of lipid peroxidation in tumor tissue, with a reduction in tumor cell proliferation ex vivo, COX-2 expression and PGE2 production. Thus, FO may act simultaneously through multiple effects to reduce tumor growth. Whether these effects are connected through a single underlying mechanism remains to be seen.
113-120
Mund, Rogéria C.
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Pizato, Nathalia
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Bonatto, Sandro
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Nunes, Everson A.
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Vicenzi, Thiago
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Tanhoffer, Ricardo
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de Oliveira, Heloisa H.P.
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Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
February 2007
Mund, Rogéria C.
e347fcef-0f67-422c-ab6f-1105dc0c9335
Pizato, Nathalia
506fb966-a881-4a09-a467-2df7b07995c5
Bonatto, Sandro
b6deac87-744a-4bdc-aa63-c739ad06cf20
Nunes, Everson A.
62a7f5db-40d7-4814-a1d0-2c1eb71a9c29
Vicenzi, Thiago
e1178583-4ead-465d-8bce-dc2f9e2f7414
Tanhoffer, Ricardo
600ec356-f431-4daa-85b6-97b6a5325502
de Oliveira, Heloisa H.P.
46133976-81c3-4c8c-b498-96b96664776a
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Fernandes, Luiz C.
380a7161-4e23-40d3-afde-7cc36ec65060
Mund, Rogéria C., Pizato, Nathalia, Bonatto, Sandro, Nunes, Everson A., Vicenzi, Thiago, Tanhoffer, Ricardo, de Oliveira, Heloisa H.P., Curi, Rui, Calder, Philip C. and Fernandes, Luiz C.
(2007)
Decreased tumor growth in Walker 256 tumor-bearing rats chronically supplemented with fish oil involves COX-2 and PGE2 reduction associated with apoptosis and increased peroxidation.
Prostaglandins, Leukotrienes and Essential Fatty Acids, 76 (2), .
(doi:10.1016/j.plefa.2006.11.008).
Abstract
Many studies have shown that addition of fish oil (FO) to the diet reduces tumor growth but the mechanism(s) of action involved is (are) still unknown. In this study, we examine some possible mechanisms in tumor-bearing rats chronically supplemented with FO. Male Wistar rats (21 days old) were fed with regular chow and supplemented with coconut or FO (1g/kg body weight) until they reached 70 days of age. Then, they were inoculated with a suspension of Walker 256 ascitic tumor cells (2 x 10(7)ml) and after 14 days they were killed. Supplementation with FO resulted in significantly lower tumor weight, greater tumor cell apoptosis, lower ex vivo tumor cell proliferation, a higher tumor content of lipid peroxides, lower expression of cyclooxygenase-2 (COX-2) in tumor tissue and a lower plasma concentration of prostaglandin E2 than observed in rats fed regular chow or supplemented with coconut oil. These results suggest that reduction of tumor growth by FO involves an increase in apoptosis and of lipid peroxidation in tumor tissue, with a reduction in tumor cell proliferation ex vivo, COX-2 expression and PGE2 production. Thus, FO may act simultaneously through multiple effects to reduce tumor growth. Whether these effects are connected through a single underlying mechanism remains to be seen.
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Published date: February 2007
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Local EPrints ID: 61392
URI: http://eprints.soton.ac.uk/id/eprint/61392
PURE UUID: 69d2e2fc-2758-4f65-86f9-865c981ff54f
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Date deposited: 30 Sep 2008
Last modified: 16 Mar 2024 02:51
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Author:
Rogéria C. Mund
Author:
Nathalia Pizato
Author:
Sandro Bonatto
Author:
Everson A. Nunes
Author:
Thiago Vicenzi
Author:
Ricardo Tanhoffer
Author:
Heloisa H.P. de Oliveira
Author:
Rui Curi
Author:
Luiz C. Fernandes
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